Correlation Analysis of SOX9, -5, and -6 as well as COL2A1 and Aggrecan Gene Expression of Collagen I Implant-Derived and Osteoarthritic Chondrocytes
| Autor: | Manuela Berger, Florian Halbwirth, Christoph Bauer, Hannes Zwickl, Angelique Reutterer, Vivek Jeyakumar, Stefan Nehrer, Eugenia Niculescu-Morzsa |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
collagen Biomedical Engineering chondrocytes Physical Therapy Sports Therapy and Rehabilitation Osteoarthritis SOX9 Article 03 medical and health sciences 0302 clinical medicine Gene expression medicine Immunology and Allergy Autologous chondrocyte implantation Transcription factor Aggrecan transcription factor Chemistry Cartilage Anatomy medicine.disease Cell biology osteoarthritis 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis correlation Transcription Factor Gene |
| Zdroj: | Cartilage |
| ISSN: | 1947-6035 |
| Popis: | Objective Matrix-assisted autologous chondrocyte implantation is frequently applied to replace damaged cartilage in order to support tissue regeneration or repair and to prevent progressive cartilage degradation and osteoarthritis. Its application, however, is limited to primary defects and contraindicated in the case of osteoarthritis that is partially ascribed to dedifferentiation and phenotype alterations of chondrocytes obtainable from patients’ biopsies. The differentiation state of chondrocytes is reflected at the level of structural gene (COL2A1, ACAN, COL1A1) and transcription factor (SOX9, 5, 6) expression. Methods/Design We determined the mRNA abundances of COL2A1, ACAN, and COL1A1as well as SOX9, -5, and -6 of freshly isolated and passaged collagen I implant–derived and osteoarthritic chondrocytes via reverse transcription–polymerase chain reaction. Moreover, we analyzed the correlation of structural and transcription factor gene expression. Thus, we were able to evaluate the impact of the mRNA levels of transcription factors on the expression of cartilage-specific structural genes. Results Significant differences were obtained (1) for freshly isolated osteoarthritic versus collagen I implant–derived chondrocytes, (2) due to passaging of the respective cell sources, (3) for osteoarthritic versus nonosteoarthritic chondrocytes, and (4) for COL2A1 versus ACAN expression with respect to the coherence with SOX9, -5, and -6 transcript levels. Conclusion Our results might contribute to a better understanding of the transcriptional regulation of structural gene expression of chondrocytes with implications for their use in matrix-assisted autologous chondrocyte implantation. |
| Databáze: | OpenAIRE |
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