| Autor: |
Christiansen, Ailsa J, Lobo, João, Fankhauser, Christian Daniel, Rothermundt, Christian, Cathomas, Richard, Batavia, Aashil A, Grogg, Josias B, Templeton, Arnoud J, Hirschi-Blickenstorfer, Anita, Lorch, Anja, Gillessen, Silke, Moch, Holger, Beyer, Jörg, Hermanns, Thomas |
| Přispěvatelé: |
University of Zurich |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Christiansen, Ailsa J; Lobo, João; Fankhauser, Christian D; Rothermundt, Christian; Cathomas, Richard; Batavia, Aashil A; Grogg, Josias B; Templeton, Arnoud J; Hirschi-Blickenstorfer, Anita; Lorch, Anja; Gillessen, Silke; Moch, Holger; Beyer, Jörg; Hermanns, Thomas (2022). Impact of differing methodologies for serum miRNA-371a-3p assessment in stage I testicular germ cell cancer recurrence. Frontiers in oncology, 12(1056823), p. 1056823. Frontiers Research Foundation 10.3389/fonc.2022.1056823 |
| Popis: |
IntroductionCurrent evidence shows that serum miR-371a-3p can identify disease recurrence in testicular germ cell tumour (TGCT) patients and correlates with tumour load. Despite convincing evidence showing the advantages of including miR-371a-3p testing to complement and overcome the classical serum tumour markers limitations, the successful introduction of a serum miRNA based test into clinical practice has been impeded by a lack of consensus regarding optimal methodologies and lack of a universal protocol and thresholds. Herein, we investigate two quantitative real-time PCR (qRT-PCR) based pipelines in detecting disease recurrence in stage I TGCT patients under active surveillance, and compare the sensitivity and specificity for each method.MethodsSequential serum samples collected from 33 stage I TGCT patients undergoing active surveillance were analysed for miR-371a-3p via qRT-PCR with and without an amplification step included.ResultsUsing a pre-amplified protocol, all known recurrences were detected via elevated miR-371a-3p expression, while without pre-amplification, we failed to detect recurrence in 3/10 known recurrence patients. For pre-amplified analysis, sensitivity and specificity was 90% and 94.4% respectively. Without amplification, sensitivity dropped to 60%, but exhibited 100% specificity.DiscussionWe conclude that incorporating pre-amplification increases sensitivity of miR-371a-3p detection, but produces more false positive results. The ideal protocol for quantification of miR-371a-3p still needs to be determined. TGCT patients undergoing active surveillance may benefit from serum miR-371a-3p quantification with earlier detection of recurrences compared to current standard methods. However, larger cross-institutional studies where samples are processed and data is analysed in a standardised manner are required prior to its routine clinical implementation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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