Comparative chromatography of plasma and plasma concentrates rich in factors VIIIand IX
| Autor: | Raymond Losito, Edith Lemieux, Bernard Longpré |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Optical Rotation
Immunoelectrophoresis Fibrinogen Biochemistry Chromatography DEAE-Cellulose Analytical Chemistry Factor IX medicine Chemical Precipitation Humans Serum Albumin Chromatography Factor VIII biology medicine.diagnostic_test Chemistry Organic Chemistry Complement Fixation Tests Albumin Ceruloplasmin General Medicine Cold Temperature Coagulation Cryoprecipitate Immunoglobulin G biology.protein medicine.drug |
| Zdroj: | Journal of chromatography. 101(2) |
| Popis: | A method for the comparison of plasma concentrates rich in the coagulation factors VIII (Hemophil™ and Cryoprecipitate) and IX (Konyne™) is presented employing DEAE-cellulose chromatography and immunoelectrophoresis. Whereas complement C-3 and ceruloplasmin was found to be strongly present in the Konyne™ preparation, this was not found to be the case in the factor VIII preparations. Both albumin and IgG were established to be present in all the concentrates; albumin was assigned to the latter parts of the chromatogram whereas IgG was found in all fractions tested. It was again shown that fibrinogen was a contaminant of the factor VIII preparations; Konyne™ was found to be free of fibrinogen in our experiments. Procoagulant activity could be isolated in some of the fractions from the column for the commercial preparations; this was not so for the other samples chromatographed. |
| Databáze: | OpenAIRE |
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