Characterization of an endo‐beta‐1,4 glucanase gene from paper‐degrading and denim bio‐stoning cellulase producing Aspergillus isolates

Autor: Jahed Ahmed, Asma‐Ul‐ Taslim, Topu Raihan, Md. Mehadi Hasan Sohag, Mahmudul Hasan, Sabrina Suhani, Firdausi Qadri, Abul Kalam Azad
Rok vydání: 2022
Předmět:
Zdroj: Biotechnology and Applied Biochemistry.
ISSN: 1470-8744
0885-4513
DOI: 10.1002/bab.2420
Popis: Cellulases are used in textile, pulp and paper, brewery and wine, sugars and ethanol industries. Four fungal isolates obtained from organic municipal solid wastes (OMSW) were selected based on their cellulolytic activity on carboxymethyl cellulose (CMC) agar medium. Based on the internal transcribed spacer (ITS) sequence of the ribosomal DNA, the four cellulolytic isolates were identified as Aspergillus fumigatus AKAL1, Aspergillus oryzae AKAL4, Aspergillus flavus AKAL8 and Aspergillus flavus AKAL9. After 9 days of fermentation at 30°C and pH 6.5 under 110 rpm agitation, these isolates produced the maximum amount of cellulase. The cellulase showed optimum activity at temperature 35-40°C and pH 6.0-7.0, and was stable for 1 h at 25-45°C and pH 5.0-7.0. The Mg
Databáze: OpenAIRE
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