The value of in vitro cell culture of granulocytes in the detection of Ehrlichia
| Autor: | Alex Mutani, James Stwart Kaminjolo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
biology medicine.drug_class Ehrlichia Cell Culture Antibiotics biology.organism_classification Virology Peripheral blood mononuclear cell Microbiology Infectious Diseases Mononuclear cells Cell culture medicine Homologous chromosome Animals Cattle Parasitology Asymptomatic carrier Cells Cultured In vitro cell culture Granulocytes Whole blood |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical v.34 n.4 2001 Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
| Popis: | Peripheral blood leukocytes from different animals were isolated from whole blood and maintained in Dulbeco's medium containing homologous serum without antibiotics. After 72 hrs microscopic examination of these cells showed that most animals were infected with Ehrlichia. Observation of thin blood smears from the same animals showed that only two were positive for Ehrlichia. The results of this investigation show that leukocyte culture is superior to the traditional thin blood film method in the detection of Ehrlichia and that asymptomatic carriers are easily detected. The method is inexpensive and does not require specific cell lines although it is necessary to use sterile sera. |
| Databáze: | OpenAIRE |
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