Long-term STED imaging of amyloid fibers with exchangeable Thioflavin T
| Autor: | Joaquim Torra, Patricia Bondia, Begoña Sot, Cristina Flors, Sylvia Gutierrez-Erlandsson |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España) |
| Rok vydání: | 2020 |
| Předmět: |
Amyloid
Fluorophore Chemistry STED microscopy 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences chemistry.chemical_compound Amyloid disease Microscopy Fluorescence mental disorders Biophysics General Materials Science Thioflavin Benzothiazoles 0210 nano-technology Amyloid fibers Fluorescent Dyes |
| Zdroj: | Repositorio Institucional del Instituto Madrileño de Estudios Avanzados en Nanociencia instname Digital.CSIC. Repositorio Institucional del CSIC |
| Popis: | We report the use of the amyloid probe Thioflavin T (ThT) as a specific and exchangeable fluorophore for stimulated emission depletion (STED) super-resolution imaging of amyloid fibers. This method achieves a spatial resolution in the range of 60–70 nm, low image background and increased photostability that enables long-term STED imaging. These results expand the widespread uses of ThT and can be potentially extended to other common amyloid fluorescent probes, providing new tools for the study of amyloid diseases. This research was supported by the Ministerio de Ciencia, Innovación y Universidades through projects PCI2018-093064 and PGC2018-094802-B-I00 (C. F.), SAF2017-87305-R (B. S.) and SEV-2016-0686 (IMDEA Nanociencia), and the Comunidad Autónoma de Madrid project S2017/BMD-3867 (C.F.). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|