A new CD44V3-containing isoform is involved in tumor cell growth and migration during human breast carcinoma progression
| Autor: | Lilly Y.W. Bourguignon, Eric D. Kalish, Naoko Iida, Frederick L. Moffat |
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| Rok vydání: | 1999 |
| Předmět: |
Gene isoform
Oncology medicine.medical_specialty Cell CA 15-3 Breast Neoplasms Biology Transfection Exon Cell Movement Internal medicine medicine Carcinoma Tumor Cells Cultured Humans Cell adhesion molecule Reverse Transcriptase Polymerase Chain Reaction Cancer medicine.disease Blot Blotting Southern medicine.anatomical_structure Hyaluronan Receptors Microscopy Fluorescence Lymphatic Metastasis Axilla Cancer research Disease Progression Female Cell Division |
| Zdroj: | Frontiers in bioscience : a journal and virtual library. 4 |
| ISSN: | 1093-9946 |
| Popis: | CD44 isoforms belong to a family of cell adhesion molecules expressed on the cell surface of many tumor cells during human breast cancer progression. In this study we have analyzed the expression of CD44v3-containing isoforms [containing heparan sulfate addition sites for growth factor binding] in primary breast tumors, axillary nodal metastases and normal breast tissue. Using reverse transcriptase-polymerase chain reaction (RT-PCR) followed by Southern blot, cloning, nucleotide sequencing and RT-in situ-PCR analyses, we have found that at least two CD44v3-containing isoforms, including one new species of CD44v2,deltav3-10 (deltav3 defined as a v3 exon lacking the first 24 base pairs) and another previously reported CD44v3,8-10 are preferentially expressed in human primary breast tumor and axillary nodal metastases but not in normal breast tissues. These finding suggest that these CD44v3-containing isoforms are closely associated with breast cancer metastasis. |
| Databáze: | OpenAIRE |
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