Transcriptional Regulation of CD1D1 by Ets Family Transcription Factors
| Autor: | Kevin Barton, Peter Laslo, Chyung Ru Wang, Yanbiao Geng |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cell type
Molecular Sequence Data Immunology B-Lymphocyte Subsets CD1 Down-Regulation Biology Cell Line Antigens CD1 Mice Cell Line Tumor Proto-Oncogene Proteins Consensus Sequence MHC class I Transcriptional regulation Animals Immunology and Allergy RNA Messenger Promoter Regions Genetic Transcription factor Gene Oligonucleotide Array Sequence Analysis Mice Knockout Binding Sites Base Sequence Proto-Oncogene Proteins c-ets Leukemia P388 Cell Membrane Ephrin-A2 Natural killer T cell Molecular biology Gene Expression Regulation Neoplastic Mice Inbred C57BL Trans-Activators biology.protein Antigens CD1d Transcription Initiation Site Chromatin immunoprecipitation Spleen Transcription Factors |
| Zdroj: | The Journal of Immunology. 175:1022-1029 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | CD1 molecules are MHC class I-like glycoproteins specialized in presenting lipid/glycolipid Ags to T cells. The distinct cell-type specific expression of CD1D1 plays an important role in the development and function of NKT cells, a unique subset of immunoregulatory T cells. However, the mechanisms regulating CD1D1 expression are largely unknown. In this study, we have characterized the upstream region of the CD1D1 gene and identified a minimal promoter region within 200 bp from the translational start site of CD1D1 that exhibits cell-type specific promoter activity. Analysis of this region revealed an Ets binding site critical for CD1D1 promoter activity. Gel shift assays and chromatin immunoprecipitation experiments showed that Elf-1 and PU.1 bind to the CD1D1 promoter. Furthermore, we found that gene disruption of Elf-1 resulted in decreased CD1D1 expression on B cells but not other cell types, whereas conditional activation of PU.1 negatively regulated CD1D1 expression in PU.1-deficient myeloid cells. These findings are the first to demonstrate that Ets proteins are involved in the transcriptional regulation of CD1D1 and that they may function uniquely in different cell types. |
| Databáze: | OpenAIRE |
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