Serum after partial hepatectomy stimulates iNOS gene transcription via downstream NF-kappa B site
| Autor: | Jae Heun Lee, Sang Gi Paik, Young Sue Ryu, Jin Young Bae, Yong Man Kim, So Hyun Bae, Gang Min Hur, Jeong Ho Seok, Jang Hee Hong |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Transcriptional Activation
P50 Biophysics Nitric Oxide Synthase Type II Response Elements Biochemistry Antibodies Cell Line Rats Sprague-Dawley Mice Animals Hepatectomy RNA Messenger Promoter Regions Genetic Molecular Biology Reporter gene biology Tumor Necrosis Factor-alpha Liver cell NF-kappa B Cell Biology NFKB1 Molecular biology Liver regeneration Culture Media Liver Regeneration Rats Nitric oxide synthase B vitamins Kinetics Blood Cell culture biology.protein Nitric Oxide Synthase |
| Zdroj: | Biochemical and biophysical research communications. 284(3) |
| ISSN: | 0006-291X |
| Popis: | It has been known that the expression of inducible nitric oxide synthase (iNOS) is up-regulated during hepatic regeneration. The present study characterized the molecular mechanisms involved in the transcriptional activation of iNOS gene by using the serum after partial hepatectomy (post-PH serum) in vitro. The post-PH serum rapidly induced iNOS mRNA expression, which was blocked by anti-tumor necrosis factor-alpha (TNF-alpha) antibody in BNL CL.2 cells, murine embryonic liver cell line. In addition, EMSAs using a NF-kappa B-specific oligomer showed that the up-regulated iNOS mRNA expression in cells treated with post-PH serum correlated with transient activation of NF-kappa B complex (p50/p65 heterodimer). Transient transfection of BNL CL.2 cells with iNOS promoter linked to a CAT reporter gene showed the transcriptional activation of iNOS promoter by post-PH serum. Furthermore, site-directed mutational analysis of the two NF-kappa B sites individually or in combination revealed that iNOS expression by post-PH serum is regulated by the downstream NF-kappa B site, but not by upstream NF-kappa B site. Taken together, these results suggest that the downstream NF-kappa B site acts as an essential component for the iNOS expression by post-PH serum during hepatic regeneration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect