Denervation causes fiber atrophy and myosin heavy chain co-expression in senescent skeletal muscle
| Autor: | Sharon L. Rowan, Douglas M. Turnbull, Karolina A. Rygiel, Fennigje M. Purves-Smith, Nathan M. Solbak, Russell T. Hepple |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Aging Anatomy and Physiology Muscle Fibers Skeletal lcsh:Medicine Muscle Proteins Cell Count Sodium Channels NAV1.5 Voltage-Gated Sodium Channel Tripartite Motif Proteins 0302 clinical medicine Myosin Myocyte lcsh:Science Musculoskeletal System Denervation Motor Neurons 0303 health sciences Multidisciplinary Organ Size Muscle atrophy medicine.anatomical_structure Spinal Cord Muscle Medicine Female medicine.symptom Research Article Nervous System Physiology medicine.medical_specialty Proteasome Endopeptidase Complex Ubiquitin-Protein Ligases Biology Neurological System Muscle Types 03 medical and health sciences Gastrocnemius muscle Atrophy Internal medicine medicine Animals 030304 developmental biology Motor Systems SKP Cullin F-Box Protein Ligases Myosin Heavy Chains lcsh:R Skeletal muscle Spinal cord medicine.disease Rats Endocrinology Gene Expression Regulation Proteolysis lcsh:Q Physiological Processes 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 1, p e29082 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Although denervation has long been implicated in aging muscle, the degree to which it is causes the fiber atrophy seen in aging muscle is unknown. To address this question, we quantified motoneuron soma counts in the lumbar spinal cord using choline acetyl transferase immunhistochemistry and quantified the size of denervated versus innervated muscle fibers in the gastrocnemius muscle using the in situ expression of the denervation-specific sodium channel, Nav1.5, in young adult (YA) and senescent (SEN) rats. To gain insights into the mechanisms driving myofiber atrophy, we also examined the myofiber expression of the two primary ubiquitin ligases necessary for muscle atrophy (MAFbx, MuRF1). MN soma number in lumbar spinal cord declined 27% between YA (638±34 MNs×mm−1) and SEN (469±13 MNs×mm−1). Nav1.5 positive fibers (1548±70 μm2) were 35% smaller than Nav1.5 negative fibers (2367±78 μm2; P |
| Databáze: | OpenAIRE |
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