Potentiometric biosensor for determination of urea in milk using immobilized Arthrobacter creatinolyticus urease
| Autor: | Jitendra Kumar, Marichetti Kuppuswami Gowthaman, Stanislaus F. D'Souza, Numbi Ramudu Kamini, Rajendran Ramesh, Puhazhselvan Puhazhendi |
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| Rok vydání: | 2015 |
| Předmět: |
Urease
Polymers Potentiometric titration Bioengineering Biosensing Techniques Methylmethacrylate Biomaterials chemistry.chemical_compound Animals Urea Arthrobacter Electrodes Ammonium sulfate precipitation Chromatography Acrylonitrile biology Temperature Enzymes Immobilized Milk Membrane chemistry Glutaral Mechanics of Materials Potentiometry biology.protein Glutaraldehyde Biosensor |
| Zdroj: | Materials Science and Engineering: C. 49:786-792 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2015.01.048 |
| Popis: | The extracellular urease from Arthrobacter creatinolyticus was partially purified by ammonium sulfate precipitation and immobilized on PAN [poly(acrylonitrile–methylmethacrylate–sodium vinylsulfonate)] membrane. The urease immobilized PAN membrane exhibited an activity of 97.92 U/cm 2 under the optimum conditions of 1.0% enzyme concentration, 15% glutaraldehyde, 24 h immobilization time and temperature of 4 °C. The changes in surface morphology of the membrane after immobilization were studied by SEM and ATR-FTIR analysis. Immobilized membrane was associated with potentiometric electrode for calibration of biosensor and the results showed a linear response for wide range of urea concentration from 1 to 100 mM. The immobilized urease had good storage stability for a period of 70 days at 4 °C and could be effectively reused for 13 cycles. Urease immobilized membrane was also employed in analysis of urea spiked milk samples. |
| Databáze: | OpenAIRE |
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