Probiotic based-diet effect on the immune response and induced stress in irradiated mass reared Ceratitis capitata males (Diptera: Tephritidae) destined for the release in the sterile insect technique programs
Autor: | Kamel Charaabi, Salma Fadhl, Haytham Hamden, Amor Mosbah, Meriem Msaad Guerfali, Wafa Djobbi, Ameur Cherif |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Male
Life Cycles Physiology Statistics as Topic Gene Expression law.invention Sterile insect technique Probiotic Larvae law Medicine and Health Sciences Food science Cobalt Radioisotopes Larva Multidisciplinary Pupa Eukaryota Agriculture Enterobacter Ceratitis capitata Insects Physiological Parameters Medicine Insect Pests Insect Proteins Female Research Article Arthropoda Science Biology Microbiology Pests Stress Physiological Tephritidae Genetics Animals Pest Control Biological Infertility Male Nutrition Probiotics fungi Body Weight Organisms Immunity Biology and Life Sciences Medfly Pupae Sterilization (microbiology) biology.organism_classification Invertebrates Diet Cecropin Gene Expression Regulation Flight Animal Zoology Entomology Developmental Biology |
Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 9, p e0257097 (2021) |
ISSN: | 1932-6203 |
Popis: | Ceratitis capitata (medfly) is one of the most devastating crop pests worldwide. The Sterile Insect Technique (SIT) is a control method that is based on the mass rearing of males, their sterilization, and release in the field. However, the effectiveness of the technique depends on the quality of the released males and their fitness. We previously isolated and selected a probiotic bacteria (Enterobacter sp.), from wild-caught medflies, according to criteria that improved biological quality traits of reared medfly males.We firstly evaluated the impact of the irradiation on the expression of different immune and stress genes in the medfly sterile males. Expression was measured at differents time points ranging from 0 to 168 h after irradiation to capture the response of genes with distinct temporal expression patterns. Then, we supplemented the larval diet with previously isolated Enterobacter sp.strain, live and autoclaved at various concentrations to see whether the probiotic treatments affect, through their protective role, the gene expression level, and quality traits. The irradiation had significant effect on the genes attacin, cecropin, PGPR-LC, hsp23, and hsp70 level expression. The expression of attacin and PGPR-LC was up-regulated while that of cecropin was down-regulated. Hsp genes showed decreased levels between 0 and 18 h to peak at 72 h. However, the supplementation of the probiotic strain, either live or autoclaved, was statistically significant only for attacingene. However, significant interaction time x probiotic was noticed for attacin, cecropin, hsp23 and hsp70. The probiotic treatments also improved the quality control parameters like pupal weight. From this work we can conclude that a consortium of parabiotics (autoclaved probiotics) treatment will be recommended in insectaries considering both the beneficial effects on mass reared insects and its general safety for insectary workers and for environment. |
Databáze: | OpenAIRE |
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