The Arabidopsis outward K+ channel GORK is involved in regulation of stomatal movements and plant transpiration
| Autor: | Hervé Sentenac, Karine Mouline, Thierry Simonneau, Eric Hosy, Frédéric Gaymard, Anne Lebaudy, Fabien Porée, Jean-Baptiste Thibaud, Jossia Boucherez, Alain Vavasseur, David Bouchez, Anne-Aliénor Véry, Ingo Dreyer |
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| Přispěvatelé: | Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Unité de recherche Génétique et amélioration des plantes (GAP), Institut National de la Recherche Agronomique (INRA), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro) |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
0106 biological sciences
Patch-Clamp Techniques Potassium Channels THALIANA ABA brassica Xenopus crucifere Turgor pressure Mutant Arabidopsis gène gork stomate 01 natural sciences cellule de garde transpiration Guard cell canal à potassium ComputingMilieux_MISCELLANEOUS Transpiration 0303 health sciences Multidisciplinary biology food and beverages Biological Sciences Potassium channel Recombinant Proteins canal à ion commelina communis l dicotyledon DNA Bacterial DNA Complementary [SDV.BC]Life Sciences [q-bio]/Cellular Biology Photosynthesis 03 medical and health sciences Botany Animals kat1 vicia faba 030304 developmental biology lumière Ion Transport Epidermis (botany) fungi arabidopsis thaliana Water biology.organism_classification régulation osmotique Commentary Biophysics Potassium 010606 plant biology & botany |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America 9 (100), 5549-5554. (2003) Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2003, 100 (9), pp.5549-5554. ⟨10.1073/pnas.0733970100⟩ |
| ISSN: | 0027-8424 1091-6490 |
| DOI: | 10.1073/pnas.0733970100⟩ |
| Popis: | Microscopic pores present in the epidermis of plant aerial organs, called stomata, allow gas exchanges between the inner photosynthetic tissue and the atmosphere. Regulation of stomatal aperture, preventing excess transpirational vapor loss, relies on turgor changes of two highly differentiated epidermal cells surrounding the pore, the guard cells. Increased guard cell turgor due to increased solute accumulation results in stomatal opening, whereas decreased guard cell turgor due to decreased solute accumulation results in stomatal closing. Here we provide direct evidence, based on reverse genetics approaches, that the Arabidopsis GORK Shaker gene encodes the major voltage-gated outwardly rectifying K + channel of the guard cell membrane. Expression of GORK dominant negative mutant polypeptides in transgenic Arabidopsis was found to strongly reduce outwardly rectifying K + channel activity in the guard cell membrane, and disruption of the GORK gene (T-DNA insertion knockout mutant) fully suppressed this activity. Bioassays on epidermal peels revealed that disruption of GORK activity resulted in impaired stomatal closure in response to darkness or the stress hormone azobenzenearsonate. Transpiration measurements on excised rosettes and intact plants (grown in hydroponic conditions or submitted to water stress) revealed that absence of GORK activity resulted in increased water consumption. The whole set of data indicates that GORK is likely to play a crucial role in adaptation to drought in fluctuating environments. |
| Databáze: | OpenAIRE |
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