Nanoplasmid Vectors Co-expressing Innate Immune Agonists Enhance DNA Vaccines for Venezuelan Equine Encephalitis Virus and Ebola Virus
| Autor: | Kristin Spik, Lesley C. Dupuy, John J. Suschak, Steven A. Kwilas, Charles J. Shoemaker, James A. Williams, Connie S. Schmaljohn, Carolyn Six |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 medicine.medical_treatment Nanoplasmid Alphavirus medicine.disease_cause DNA vaccination RIG-I 03 medical and health sciences 0302 clinical medicine Plasmid CpG Genetics medicine DNA Vaccine lcsh:QH573-671 Innate Immune Agonist Molecular Biology Ebola virus Innate immune system biology lcsh:Cytology Immunogenicity biology.organism_classification Virology Genetic Adjuvant lcsh:Genetics 030104 developmental biology 030220 oncology & carcinogenesis Venezuelan equine encephalitis virus Molecular Medicine Adjuvant |
| Zdroj: | Molecular Therapy: Methods & Clinical Development, Vol 17, Iss, Pp 810-821 (2020) |
| ISSN: | 2329-0501 |
| DOI: | 10.1016/j.omtm.2020.04.009 |
| Popis: | DNA vaccines expressing codon-optimized Venezuelan equine encephalitis virus (VEEV) and Ebola virus (EBOV) glycoprotein genes provide protective immunity to mice and nonhuman primates when delivered by intramuscular (IM) electroporation (EP). To achieve equivalent protective efficacy in the absence of EP, we evaluated VEEV and EBOV DNA vaccines constructed using minimalized Nanoplasmid expression vectors that are smaller than conventional plasmids used for DNA vaccination. These vectors may also be designed to co-express type I interferon inducing innate immune agonist genes that have an adjuvant effect. Nanoplasmid vaccinated mice had increased antibody responses as compared to those receiving our conventional pWRG7077-based vaccines when delivered by IM injection, and these responses were further enhanced by the inclusion of the innate immune agonist genes. The Nanoplasmid VEEV DNA vaccines also significantly increased protection against aerosol VEEV challenge as compared to the pWRG7077 VEEV DNA vaccine. Although all mice receiving the pWRG7077 and Nanoplasmid EBOV DNA vaccines at the dose tested survived EBOV challenge, only mice receiving the Nanoplasmid EBOV DNA vaccine that co-expresses the innate immune agonist genes failed to lose weight after challenge. Our results suggest that Nanoplasmid vectors can improve the immunogenicity and protective efficacy of alphavirus and filovirus DNA vaccines. |
| Databáze: | OpenAIRE |
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