Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids
| Autor: | Ran Tao, Sana Rauf, Lei Yang, Honglang Li, Jingting Luo, Hafiz Imran Ahmad Qazi, Chen Fu, Yongqing Fu, Sajid Rauf |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Detection limit
immunosensor Chromatography Materials science biology Biocompatibility gold staining F100 Surface acoustic wave AFP Nanoparticle H800 QD415-436 Primary and secondary antibodies Biochemistry Analytical Chemistry Specific surface area Biological fluids biology.protein leaky surface acoustic wave (LSAW) Physical and Theoretical Chemistry Alpha-fetoprotein MoS2@Cu2O-Au |
| Zdroj: | Chemosensors, Vol 9, Iss 311, p 311 (2021) Chemosensors Volume 9 Issue 11 |
| ISSN: | 2227-9040 |
| Popis: | We propose an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide @ cuprous oxide—gold (MoS2@Cu2O-Au) nanoparticles and subsequent gold staining for the detection of alpha-fetoprotein (AFP). MoS2@Cu2O-Au nanoparticles, with their large specific surface area and good biocompatibility, not only capture the secondary antibodies (Ab2) but also amplify the mass loading effect of the acoustic wave sensor in the detection of AFP. The immunosensor signals are further amplified upon injection of gold staining solution. The developed immunosensor achieved a low detection limit of 5.5 and 25.0 pg/mL with and without gold staining, respectively. The immunosensor demonstrated its efficiency for the quantitative detection of AFP in complex biological fluids, including human serum and saliva samples, with excellent selectivity and long-term stability, showing great potential for the quantification of AFP in clinical diagnosis. |
| Databáze: | OpenAIRE |
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