Regional variations in the 'adenine/oxypurine' pool of the heart in normoxia and oxygen deficiency
| Autor: | D.G. Hollis, L.C. Armiger, R.N. Seelye |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Male
medicine.medical_specialty Adenosine Biology Xanthine High-performance liquid chromatography Pathology and Forensic Medicine chemistry.chemical_compound Dogs Inosine Monophosphate Internal medicine medicine Animals Hypoxia Inosine Chromatography High Pressure Liquid Hypoxanthine Adenine Nucleotides Myocardium NAD Uric Acid Endocrinology chemistry Biochemistry Hypoxanthines Xanthines Uric acid Female NAD+ kinase Electrical conduction system of the heart Energy Metabolism medicine.drug |
| Zdroj: | Experimental pathology. 30:33-38 |
| ISSN: | 0232-1513 |
| DOI: | 10.1016/s0232-1513(86)80055-x |
| Popis: | The regional distribution of energy-related metabolites was determined for the normal dog heart by using high performance liquid chromatography to analyse the levels of ATP, ADP, AMP, IMP, adenosine, inosine, hypoxanthine/xanthine, uric acid and NAD in tissue samples from 7 defined areas of the myocardium and 2 of the conduction system, and calculating the overall concentration of these metabolites in each area. From 8 of the 20 hearts used, additional samples were taken and allowed to undergo autolysis in vitro at 37 degrees C for up to 2 h before being analysed in the same manner. The total concentration of the metabolites assayed ("adenine/oxypurine" pool) did differ from one area to another, most notably between ventricles (left = 6.08, right = 5.93, apex = 6.08 microM/g wet tissue weight) and atria (left wall = 4.44, left appendage = 4.12, right wall = 4.34, right appendage = 3.57 microM/g wet tissue weight), but for each area remained essentially constant during the period of autolysis studied. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|