Identification of a Dendrimeric Heparan Sulfate-Binding Peptide That Inhibits Infectivity of Genital Types of Human Papillomaviruses
| Autor: | Donatella Allemand, Marco Rusnati, Antonella Bugatti, Andrea Giuliani, David Lembo, Manuela Donalisio, Giovanna Pirri, Andrea Civra, Santo Landolfo |
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| Rok vydání: | 2010 |
| Předmět: |
Dendrimers
Cell Survival peptide dendrimer Peptide CHO Cells Biology Antiviral Agents Virus Cell Line Inhibitory Concentration 50 chemistry.chemical_compound heparan sulfate proteoglycans antivirals Cricetulus Microscopy Electron Transmission human papilloma virus Cell Line Tumor Cricetinae Microbicide Animals Humans Pharmacology (medical) dendrimers Cytotoxicity Papillomaviridae Pharmacology chemistry.chemical_classification Infectivity Heparan sulfate Papillomavirus Surface Plasmon Resonance antiviral Molecular biology In vitro Infectious Diseases chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Heparan sulfate binding heparan sulfate Heparitin Sulfate Peptides microbicide HeLa Cells |
| Zdroj: | Antimicrobial Agents and Chemotherapy |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.00471-10 |
| Popis: | Peptide dendrimers consist of a peptidyl branching core and/or covalently attached surface functional units. They show a variety of biological properties, including antiviral activity. In this study, a minilibrary of linear, dimeric, and dendrimeric peptides containing clusters of basic amino acids was evaluated for in vitro activity against human papillomaviruses (HPVs). The peptide dendrimer SB105-A10 was found to be a potent inhibitor of genital HPV types (i.e., types 16, 18, and 6) in pseudovirus-based neutralization assays. The 50% inhibitory concentration was between 2.8 and 4.2 μg/ml (0.59 and 0.88 μM), and no evidence of cytotoxicity was observed. SB105-A10 interacts with immobilized heparin and with heparan sulfates exposed on the cell surface, most likely preventing virus attachment. The findings from this study indicate SB105-A10 to be a leading candidate compound for further development as an active ingredient of a topical microbicide against HPV and other sexually transmitted viral infections. |
| Databáze: | OpenAIRE |
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