Variation analysis of transcriptome changes reveals cochlear genes and their associated functions in cochlear susceptibility to acoustic overstimulation
| Autor: | Jonathan E. Bard, Bo Hua Hu, Qunfeng Cai, Shuzhi Yang, Weiping Yang, Jianmin Wang, Jennifer Jamison |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Macromolecular Substances Glutamic Acid RNA-Seq Biology Article Transcriptome Mice Hair Cells Auditory Gene expression Evoked Potentials Auditory Brain Stem otorhinolaryngologic diseases medicine Animals Auditory system KEGG Organ of Corti Gene Cochlea Genetics Sequence Analysis RNA Gene Expression Profiling Computational Biology Genetic Variation Ear Sensory Systems Cell biology Mice Inbred C57BL Variation (linguistics) medicine.anatomical_structure Acoustic Stimulation Hearing Loss Noise-Induced Mice Inbred CBA RNA Female sense organs Noise |
| Zdroj: | Hearing Research. 330:78-89 |
| ISSN: | 0378-5955 |
| Popis: | Individual variation in the susceptibility of the auditory system to acoustic overstimulation has been well-documented at both the functional and structural levels. However, the molecular mechanism responsible for this variation is unclear. The current investigation was designed to examine the variation patterns of cochlear gene expression using RNA-seq data and to identify the genes with expression variation that increased following acoustic trauma. This study revealed that the constitutive expressions of cochlear genes displayed diverse levels of gene-specific variation. These variation patterns were altered by acoustic trauma; approximately one-third of the examined genes displayed marked increases in their expression variation. Bioinformatics analyses revealed that the genes that exhibited increased variation were functionally related to cell death, biomolecule metabolism, and membrane function. In contrast, the stable genes were primarily related to basic cellular processes, including protein and macromolecular syntheses and transport. There was no functional overlap between the stable and variable genes. Importantly, we demonstrated that glutamate metabolism is related to the variation in the functional response of the cochlea to acoustic overstimulation. Taken together, the results indicate that our analyses of the individual variations in transcriptome changes of cochlear genes provide important information for the identification of genes that potentially contribute to the generation of individual variation in cochlear responses to acoustic overstimulation. |
| Databáze: | OpenAIRE |
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