Detection of placental transcription factor mRNA in maternal plasma
| Autor: | Allerdien Visser, Cees B.M. Oudejans, John M.G. van Vugt, Attie T. J. I. Go, Marinus A. Blankenstein, Monique A.M. Mulders |
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| Rok vydání: | 2004 |
| Předmět: |
Messenger RNA
Placenta Biochemistry (medical) Clinical Biochemistry Postpartum Period RNA Mothers Biology Y chromosome Non-coding RNA Molecular biology medicine.anatomical_structure Pregnancy Blood plasma medicine Humans Female Pregnancy Trimesters RNA Messenger Genomic imprinting Gene Transcription Factors |
| Zdroj: | Clinical chemistry. 50(8) |
| ISSN: | 0009-9147 |
| Popis: | mRNA of placental origin, including chromosome 21-encoded mRNA, can be detected reliably in maternal plasma during the first trimester of pregnancy (1)(2). The presence and detectability of placental RNA in maternal plasma permits rapid screening of new markers to test their feasibility for use in noninvasive prenatal diagnostic assays. In contrast to conventional protein-based assays, new markers can include gene products with intracellular localization and noncoding mRNA. We challenged these features by screening maternal plasma for a large number of RNA targets (n = 80) known or expected to be present in extraembryonic tissues. This set included genes coding for transcription factors, genes subject to genomic imprinting, genes coding for noncoding RNA, and other genes with restricted or abundant expression in trophoblast cells. Target genes were distributed over all chromosomes except the Y chromosome. Peripheral blood samples were collected from pregnant women attending the Prenatal Diagnostic Centre of the VU University Medical Center. All participants gave informed consent before being included in the study. The study was approved by the VU University Medical Center Ethics Committee. EDTA blood was collected between weeks 9 and 13 of pregnancy. All blood samples were obtained before invasive diagnostic procedures and processed as described previously (2). RNA was extracted from 1.6 mL of maternal plasma by silica-based affinity isolation with use of the QIAamp MinElute Virus Vacuum system (Qiagen) with minor modifications (2). The amount … |
| Databáze: | OpenAIRE |
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