Fabrication of a sensitive colorimetric nanosensor for determination of cysteine in human serum and urine samples based on magnetic-sulfur, nitrogen graphene quantum dots as a selective platform and Au nanoparticles
| Autor: | Alessandro Pedrini, Ali Mohammad Haji Shabani, Elahe Kazemi, Roya Afsharipour, Roberto Verucchi, Shayessteh Dadfarnia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Colorimetric nanosensor
Nitrogen Fe3O4/S Metal Nanoparticles Nanoparticle 02 engineering and technology 01 natural sciences N-GQDs Analytical Chemistry law.invention Absorbance Adsorption Nanosensor law Quantum Dots Humans Cysteine Surface plasmon resonance Detection limit Chemistry Graphene Magnetic Phenomena 010401 analytical chemistry technology industry and agriculture 021001 nanoscience & nanotechnology Au NPs 0104 chemical sciences Colloidal gold Colorimetry Graphite Gold 0210 nano-technology Sulfur Nuclear chemistry |
| Zdroj: | Talanta (Oxf.) 226 (2021): 122055-1. doi:10.1016/j.talanta.2020.122055 info:cnr-pdr/source/autori:Roya Afsharipour, Shayessteh Dadfarnia, Ali Mohammad Haji Shabani, Elahe Kazemi, Alessandro Pedrini, Roberto Verucchi/titolo:Fabrication of a sensitive colorimetric nanosensor for determination of cysteine in human serum and urine samples based on magnetic-sulfur, nitrogen graphene quantum dots as a selective platform and Au nanoparticles/doi:10.1016%2Fj.talanta.2020.122055/rivista:Talanta (Oxf.)/anno:2021/pagina_da:122055-1/pagina_a:/intervallo_pagine:122055-1/volume:226 |
| DOI: | 10.1016/j.talanta.2020.122055 |
| Popis: | A novel colorimetric nanosensor is reported for the selective and sensitive determination of cysteine using magnetic-sulfur, nitrogen graphene quantum dots (Fe3O4/S, N-GQDs), and gold nanoparticles (Au NPs). Thus, S, N-GQDs was firstly immobilized on Fe3O4 nanoparticles through its magnetization in the presence of Fe3+ in the alkali solution. The prepared Fe3O4/S, N-GQDs were dispersed in cysteine solution resulting in its quick adsorption on the surface of the Fe3O4/S, N-GQDs through hydrogen bonding interaction. Then, Au NPs solution was added to this mixture that after a short time, the color of Au NPs changed from red to blue, the intensity of surface plasmon resonance peak of Au NPs at 530 nm decreased, and a new peak at a higher wavelength of 680 nm appeared. The effective parameters on cysteine quantification were optimized via response surface methodology using the central composite design. Under optimum conditions, the absorbance ratio (A680/A530) has a linear proportionality with cysteine concentration in the range of 0.04–1.20 μmol L−1 with a limit of detection of 0.009 μmol L−1. The fabrication of the reported nanosensor is simple, fast, and is capable of efficient quantification of ultra traces of cysteine in human serum and urine real samples. |
| Databáze: | OpenAIRE |
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