Development of fluorescence quenching in Chlamydomonas reinhardtii upon prolonged illumination at 77 K
| Autor: | Jan P. Dekker, Joris J. Snellenburg, Ivo H. M. van Stokkum, Lucyna M. Wlodarczyk |
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| Přispěvatelé: | Biophysics Photosynthesis/Energy, LaserLaB - Energy |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chlorophyll Photosystem II Chlamydomonas reinhardtii Target analysis Plant Science Photochemistry Biochemistry Fluorescence spectroscopy Fluorescence 03 medical and health sciences Reaction rate constant Photosynthesis Quenching (fluorescence) biology Chemistry Photosystem II Protein Complex Time-resolved fluorescence Cell Biology General Medicine Nanosecond biology.organism_classification Cold Temperature 030104 developmental biology Spectrometry Fluorescence State transitions Original Article Time-resolved spectroscopy SDG 6 - Clean Water and Sanitation |
| Zdroj: | Photosynthesis Research Wlodarczyk, L M, Snellenburg, J J, Dekker, J P & Stokkum, I H M 2018, ' Development of fluorescence quenching in Chlamydomonas reinhardtii upon prolonged illumination at 77 K ', Photosynthesis Research, vol. 137, no. 3, pp. 503-513 . https://doi.org/10.1007/s11120-018-0534-8 Photosynthesis Research, 137(3), 503-513. Springer Netherlands |
| ISSN: | 1573-5079 0166-8595 |
| Popis: | Low-temperature fluorescence measurements are frequently used in photosynthesis research to assess photosynthetic processes. Upon illumination of photosystem II (PSII) frozen to 77 K, fluorescence quenching is observed. In this work, we studied the light-induced quenching in intact cells of Chlamydomonas reinhardtii at 77 K using time-resolved fluorescence spectroscopy with a streak camera setup. In agreement with previous studies, global analysis of the data shows that prolonged illumination of the sample affects the nanosecond decay component of the PSII emission. Using target analysis, we resolved the quenching on the PSII-684 compartment which describes bulk chlorophyll molecules of the PSII core antenna. Further, we quantified the quenching rate constant and observed that as the illumination proceeds the accumulation of the quencher leads to a speed up of the fluorescence decay of the PSII-684 compartment as the decay rate constant increases from about 3 to 4 ns− 1. The quenching on PSII-684 leads to indirect quenching of the compartments PSII-690 and PSII-695 which represent the red chlorophyll of the PSII core. These results explain past and current observations of light-induced quenching in 77 K steady-state and time-resolved fluorescence spectra. Electronic supplementary material The online version of this article (10.1007/s11120-018-0534-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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