Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains
| Autor: | Takashi Sugimura, Michio Miyata, Hiro Wakasugi, Masaaki Terada, Yoshinori Ikarashi, Hidenori Maruoka, Kouichi Shinohara |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.drug_class
Receptors Antigen T-Cell alpha-beta CD3 Immunology Monoclonal antibody Flow cytometry Interferon-gamma Mice Interleukin 21 T-Lymphocyte Subsets medicine Animals Immunology and Allergy Cytotoxic T cell Interferon gamma Interleukin 4 Mice Inbred C3H CD40 biology medicine.diagnostic_test Antibodies Monoclonal Flow Cytometry Molecular biology Killer Cells Natural Mice Inbred C57BL Phenotype Liver biology.protein Female Interleukin-4 medicine.drug |
| Zdroj: | European Journal of Immunology. 29:1871-1878 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/(sici)1521-4141(199906)29:06<1871::aid-immu1871>3.0.co;2-d |
| Popis: | We previously reported and partially characterized a unique monoclonal antibody (mAb), U5A2-13, which recognizes a T cell subset similar to NK1.1+ T cells, not only in NK1.1-positive mouse strains but also in NK1.1-negative strains. In NK1.1-positive C57BL/6 mice, U5A2-13+ TCRalphabeta+ cells produced abundant IL-4 as well as extremely high levels of IFN-gamma upon CD3 cross-linking, but this did not occur with U5A2-13- TCRalphabeta+ cells. In NK1.1-negative C3H/He mice, U5A2-13+ TCRalphabeta+ cells produced high levels of IL-4 and IFN-gamma upon CD3 cross-linking, but this was not observed with U5A2-13- TCRalphabeta+ cells. To the best of our knowledge, this is the first direct evidence of the presence of NK-like T cells defined phenotypically by U5A2-13 mAb and functionally by IL-4/IFN-gamma production in NK1.1-negative mouse strains. We also demonstrated that U5A2-13- NK1.1+ T cells and U5A2-13+ NK1.1- T cells in C57BL/6 mice could produce both IL-4 and IFN-gamma. In addition, Vbeta8 or Vbeta7 usage by U5A2-13+ NK1.1- T cells was lower than that by U5A2-13+ NK1.1+ T cells, but remained higher than that by U5A2-13- NK1.1- T cells. Based on the present results, U5A2-13 mAb appears to be a valuable tool in the study of NK-like T cells. |
| Databáze: | OpenAIRE |
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