Cellular distribution of pulmonary Mn and CuZn superoxide dismutase: effect of hyperoxia and interleukin-1
| Autor: | J. N. Pastore, Min-Fu Tsan, Gexin Tang, C. Y. Lee |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Cell type medicine.medical_specialty Pathology Histology Time Factors Endothelium animal diseases Biology Superoxide dismutase Rats Sprague-Dawley Internal medicine medicine Animals Respiratory system Lung Hyperoxia Superoxide Dismutase fungi Interleukin Muscle Smooth Immunohistochemistry Rats Oxygen enzymes and coenzymes (carbohydrates) medicine.anatomical_structure Endocrinology Toxicity biology.protein Anatomy medicine.symptom Interleukin-1 |
| Zdroj: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 42(9) |
| ISSN: | 0022-1554 |
| Popis: | Pulmonary superoxide dismutase (SOD) plays an important role in the lung defense against O2 toxicity. We have previously demonstrated that tracheal insufflation of interleukin-1 alpha (IL-1) selectively enhances pulmonary MnSOD and protects rats against O2 toxicity. However, little is known about the cellular distribution of pulmonary MnSOD- and CuZnSOD-specific proteins. We performed immunohistochemistry in plastic sections (2 microns thick) to determine the effects of hyperoxia and IL-1 on the cellular distribution of pulmonary MnSOD and CuZnSOD in rats. MnSOD and CuZnSOD were present in all lung cells. Smooth muscle and endothelial cells appeared to contain higher immunoreactive MnSOD and CuZnSOD proteins than other lung cell types. Exposure of rats to 100% O2 for 24 hr had no effect on the cellular distribution and intensity of pulmonary MnSOD. However, at 50 hr after O2 exposure the intensity of pulmonary MnSOD was reduced. In contrast, tracheal insufflation of IL-1 markedly enhanced the intensity of pulmonary MnSOD in rats exposed to O2 for 50 hr. Neither O2 exposure nor IL-1 insufflation had any apparent effect on the distribution and intensity of pulmonary CuZnSOD. We conclude that IL-1 selectively enhances pulmonary MnSOD and that this effect is manifested in most lung cells, particularly smooth muscle and endothelial cells. |
| Databáze: | OpenAIRE |
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