Oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells express glutamine synthetase: developmental and cell type-specific regulation
| Autor: | D. Dalençon, C. Fressinaud, L Vitkovic, Sarlieve Ll, Dominique Baas |
|---|---|
| Přispěvatelé: | Deleage, Gilbert |
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Cell
Biology Gene Expression Regulation Enzymologic Rats Sprague-Dawley Cellular and Molecular Neuroscience Glutamate-Ammonia Ligase Glutamine synthetase Gene expression medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals Progenitor cell Molecular Biology Cells Cultured chemistry.chemical_classification Stem Cells Brain Gene Expression Regulation Developmental Molecular biology Oligodendrocyte Rats Psychiatry and Mental health Oligodendroglia medicine.anatomical_structure Enzyme chemistry Animals Newborn Astrocytes Neuroglia Astrocyte |
| Popis: | Glutamine synthetase (GS), the enzyme that catalyses glutamine synthesis from glutamate and ammonia, plays a central role in the detoxification of brain ammonia. In the central nervous system (CNS), GS also subserves additional important functions such as regulating glutamate, GABA and amino acid metabolism. Oligodendrocytes (OL) form the myelin sheath in the central nervous system (CNS) and are essential for efficient propagation of nerve impulses. In culture, OL arise from bipotential O-2A progenitor cells. These O-2A cells give rise to type-2 astrocytes in the presence of serum. GS is expressed in mature glial cells in vivo and in vitro, but it is unknown whether GS is present in glial progenitors. In addition, a comparison of the GS expression level among the various types of glial cells has never been done in vitro. The current study investigates in vitro GS expression levels in O-2A progenitors, astrocytes and OL. We demonstrate that the GS gene is expressed in O-2A progenitors and is expressed at different levels in each cultured glial cell type. GS also is stimulated during OL developmental maturation. Thus, the GS gene is expressed in O-2A cells and is regulated in a developmental and macroglial cell type-specific manner.Glutamine synthetase (GS), the enzyme that catalyses glutamine synthesis from glutamate and ammonia, plays a central role in the detoxification of brain ammonia. In the central nervous system (CNS), GS also subserves additional important functions such as regulating glutamate, GABA and amino acid metabolism. Oligodendrocytes (OL) form the myelin sheath in the central nervous system (CNS) and are essential for efficient propagation of nerve impulses. In culture, OL arise from bipotential O-2A progenitor cells. These O-2A cells give rise to type-2 astrocytes in the presence of serum. GS is expressed in mature glial cells in vivo and in vitro, but it is unknown whether GS is present in glial progenitors. In addition, a comparison of the GS expression level among the various types of glial cells has never been done in vitro. The current study investigates in vitro GS expression levels in O-2A progenitors, astrocytes and OL. We demonstrate that the GS gene is expressed in O-2A progenitors and is expressed at different levels in each cultured glial cell type. GS also is stimulated during OL developmental maturation. Thus, the GS gene is expressed in O-2A cells and is regulated in a developmental and macroglial cell type-specific manner. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|