Sequential FISH analysis of oocytes and polar bodies reveals aneuploidy mechanisms

Autor: CM Conn, K.M. Whalley, Mjw Faed, Elpida Fragouli, JA Mills, Joy D. A. Delhanty, S Cupisti
Rok vydání: 2003
Předmět:
Zdroj: Prenatal Diagnosis. 23:663-668
ISSN: 1097-0223
0197-3851
DOI: 10.1002/pd.665
Popis: Objectives Constitutional aneuploidy occurs in at least 5% of recognised pregnancies, with apparent preferential involvement of the X chromosome and the smaller autosomes. Molecular cytogenetic investigations of cleavage-stage embryos have revealed anomalies affecting all sizes of chromosomes. The aim was to investigate the variety of anomalies arising during maternal meiosis I by analysis of unfertilised oocytes and polar bodies to gain insight into aneuploidy mechanisms. Methods Sequential FISH analysis was carried out with specific probes derived from eight chromosomes, representing all sizes. Only imbalance due to a gain of a whole chromosome or chromatid, represented by extra signals, was counted to avoid artefact. Results Data were obtained on 236 eggs from 124 patients of average age 32.5 years (range 22–44). Ten patients (average 32.6 years) had abnormal eggs. The abnormality rate for oocytes and for polar bodies was close to 4% for each. Fourteen hyperploidies were found, seven involving additional single chromatids. The abnormalities affected chromosomes 13,16,18, 21 and X but not chromosomes 1, 9 or 12. Conclusion The data provide evidence for several mechanisms leading to aneuploidy, including classical non-disjunction of whole univalents; pre-division of chromatids prior to anaphase I, leading to imbalance detected at metaphase II; gonadal mosaicism for a trisomic cell line and preferential involvement of the smaller chromosomes. Monosomy for the large autosomes is not uncommon in cleavage-stage embryos and may additionally arise from anaphase lag preferentially affecting such chromosomes. Copyright © 2003 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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