Dextran: Influence of Molecular Weight in Antioxidant Properties and Immunomodulatory Potential
| Autor: | Vinícius Campelo Soeiro, Mayara Jane Campos de Medeiros, Leandro Silva Costa, Hugo Alexandre Oliveira Rocha, Maria Lara Porpino de Meiroz Grilo, Daniel de Lima Pontes, M. Alves, Karoline Rachel Teodosio Melo, Jailma Almeida-Lima |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Antioxidant medicine.medical_treatment antioxidant activity 02 engineering and technology Leuconostoc mesenteroides Antioxidants lcsh:Chemistry Lipid peroxidation chemistry.chemical_compound Mice α-d-glucans Hydrogen peroxide lcsh:QH301-705.5 Spectroscopy immunomodulatory activity 40 kDa dextran biology Superoxide Dextrans General Medicine 021001 nanoscience & nanotechnology Computer Science Applications Dextran Biochemistry 0210 nano-technology Nitric Oxide Catalysis Article Nitric oxide Inorganic Chemistry 03 medical and health sciences medicine Animals Physical and Theoretical Chemistry Molecular Biology Cell Proliferation α Molecular mass Macrophages Organic Chemistry biology.organism_classification Molecular Weight 030104 developmental biology RAW 264.7 Cells lcsh:Biology (General) lcsh:QD1-999 chemistry Lipid Peroxidation |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 17, Iss 8, p 1340 (2016) International Journal of Molecular Sciences; Volume 17; Issue 8; Pages: 1340 |
| ISSN: | 1422-0067 |
| Popis: | Dextrans (α-d-glucans) extracted from Leuconostoc mesenteroides, with molecular weights (MW) of 10 (D10), 40 (D40) and 147 (D147) kDa, were evaluated as antioxidant, anticoagulant and immunomodulatory drugs for the first time. None presented anticoagulant activity. As for the antioxidant and immunomodulatory tests, a specific test showed an increase in the dextran activity that was proportional to the increase in molecular weight. In a different assay, however, activity decreased or showed no correlation to the MW. As an example, the reducing power assay showed that D147 was twice as potent as other dextrans. On the other hand, all three samples showed similar activity (50%) when it came to scavenging the OH radical, whereas only the D10 sample showed sharp activity (50%) when it came to scavenging the superoxide ion. D40 was the single dextran that presented with immunomodulatory features since it stimulated the proliferation (~50%) of murine macrophages (RAW 264.7) and decreased the release of nitric oxide (~40%) by the cells, both in the absence and presence of lipopolysaccharides (LPS). In addition, D40 showed a greater scavenging activity (50%) for the hydrogen peroxide, which caused it to also be the more potent dextran when it came to inhibiting lipid peroxidation (70%). These points toward dextrans with a 40 kDa weight as being ideal for antioxidant and immunomodulatory use. However, future studies with the D40 and other similarly 40 kDa dextrans are underway to confirm this hypothesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|