The Arabidopsis LAZY1 Family Plays a Key Role in Gravity Signaling within Statocytes and in Branch Angle Control of Roots and Shoots
| Autor: | Toyohito Fushita, Masahiko Furutani, Masatsugu Toyota, Hirokazu Tanaka, Masatoshi Taniguchi, Moritaka Nakamura, Takeshi Nishimura, Kenichiro Baba, Miyo Terao Morita, Kohta Iijima, Masao Tasaka |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Gravitropism Arabidopsis Down-Regulation Plant Science Genes Plant Plant Roots 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Auxin Amyloplast Amino Acid Sequence Root cap Research Articles Institut für Biochemie und Biologie Statocyte chemistry.chemical_classification Genetics Indoleacetic Acids biology Arabidopsis Proteins Lateral root Biological Transport Cell Biology biology.organism_classification Cell biology 030104 developmental biology chemistry Multigene Family Mutation Polar auxin transport Plant Shoots Gravitation Signal Transduction 010606 plant biology & botany |
| Zdroj: | The Plant Cell. 29:1984-1999 |
| ISSN: | 1532-298X 1040-4651 |
| Popis: | During gravitropism, the directional signal of gravity is perceived by gravity-sensing cells called statocytes, leading to asymmetric distribution of auxin in the responding organs. To identify the genes involved in gravity signaling in statocytes, we performed transcriptome analyses of statocyte-deficient Arabidopsis thaliana mutants and found two candidates from the LAZY1 family, AtLAZY1/LAZY1-LIKE1 (LZY1) and AtDRO3/AtNGR1/LZY2. We showed that LZY1, LZY2, and a paralog AtDRO1/AtNGR2/LZY3 are redundantly involved in gravitropism of the inflorescence stem, hypocotyl, and root. Mutations of LZY genes affected early processes in gravity signal transduction without affecting amyloplast sedimentation. Statocyte-specific expression of LZY genes rescued the mutant phenotype, suggesting that LZY genes mediate gravity signaling in statocytes downstream of amyloplast displacement, leading to the generation of asymmetric auxin distribution in gravity-responding organs. We also found that lzy mutations reversed the growth angle of lateral branches and roots. Moreover, expression of the conserved C-terminal region of LZY proteins also reversed the growth direction of primary roots in the lzy mutant background. In lateral root tips of lzy multiple mutants, asymmetric distribution of PIN3 and auxin response were reversed, suggesting that LZY genes regulate the direction of polar auxin transport in response to gravity through the control of asymmetric PIN3 expression in the root cap columella. |
| Databáze: | OpenAIRE |
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