Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
| Autor: | Penney M. Gilbert, Alejandro Jurado, Mohammad Ebrahim Afshar, Roman Tsukanov, Nazar Oleksiievets, Timo Betz, Bernhard Wallmeyer, Tamara Limon, Majid Ebrahimi, Jörg Enderlein, Arne D Hofemeier, Till Moritz Muenker |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Muscle tissue Mouse QH301-705.5 Science Video microscopy Physics of Living Systems Muscle Development Regenerative medicine General Biochemistry Genetics and Molecular Biology Cell Line Mice 03 medical and health sciences 0302 clinical medicine Biomimetics medicine Animals Homeostasis Humans Biology (General) Muscle Skeletal Tissue homeostasis reconstituted muscle General Immunology and Microbiology Tension (physics) Chemistry General Neuroscience Biomechanics Skeletal muscle Cell Differentiation General Medicine Stem Cells and Regenerative Medicine Tools and Resources Biomechanical Phenomena 030104 developmental biology medicine.anatomical_structure Biophysics Medicine tension sensor 030217 neurology & neurosurgery Human |
| Zdroj: | eLife eLife, Vol 10 (2021) |
| ISSN: | 2050-084X |
| DOI: | 10.7554/elife.60145 |
| Popis: | Tension and mechanical properties of muscle tissue are tightly related to proper skeletal muscle function, which makes experimental access to the biomechanics of muscle tissue formation a key requirement to advance our understanding of muscle function and development. Recently developed elastic in vitro culture chambers allow for raising 3D muscle tissue under controlled conditions and to measure global tissue force generation. However, these chambers are inherently incompatible with high-resolution microscopy limiting their usability to global force measurements, and preventing the exploitation of modern fluorescence based investigation methods for live and dynamic measurements. Here, we present a new chamber design pairing global force measurements, quantified from post-deflection, with local tension measurements obtained from elastic hydrogel beads embedded in muscle tissue. High-resolution 3D video microscopy of engineered muscle formation, enabled by the new chamber, shows an early mechanical tissue homeostasis that remains stable in spite of continued myotube maturation. |
| Databáze: | OpenAIRE |
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