Primary Structure of Somatostatin, A Hypothalamic Peptide That Inhibits the Secretion of Pituitary Growth Hormone
| Autor: | Roger Guillemin, Nicholas Ling, Madalyn Butcher, Roger Burgus |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
medicine.medical_specialty
Pituitary gland Hypothalamus Peptide Biology Mass Spectrometry Internal medicine medicine Animals Chemical Precipitation Chymotrypsin Trypsin Amino Acid Sequence Peptide sequence chemistry.chemical_classification Autoanalysis Sheep Multidisciplinary Edman degradation Tissue Extracts Protein primary structure Somatostatin Endocrinology medicine.anatomical_structure chemistry Biochemistry Growth Hormone Pituitary Gland Biological Sciences: Biochemistry Peptides medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 70:684-688 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.70.3.684 |
| Popis: | Somatostatin, a peptide isolated from ovine hypothalamic tissue that inhibits the release of radioimmunoassayable growth hormone in vitro from rat or human pituitary cells or in vivo in rats, has the primary structure [Formula: see text]. The structure was established by submitting the carboxymethylated peptide, the carboxymethylated tryptic digest, and the chymotryptic digest of the peptide to Edman degradation. Degradation products were analyzed by amino-acid analysis, as well as in some cases by determination of N-termini by dansylation or by determination of phenylthiohydantoins by mass spectrometry. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|