Further evidence for microtubule-independent dimerization of TPPP/p25
| Autor: | Judit Ovádi, Judit Oláh, Attila Lehotzky, Sándor Szunyogh, Tibor Szénási, Adél Szabó |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Molecular model Intracellular Space Nerve Tissue Proteins Microtubules Models Biological Fluorescence Article Polymerization Microbiology 03 medical and health sciences Bimolecular fluorescence complementation TUBULIN POLYMERIZATION-PROMOTING PROTEIN Nephelometry and Turbidimetry Tubulin Microtubule Animals Humans Multidisciplinary Chemistry Oligodendrocyte differentiation Cell biology Cytosol 030104 developmental biology Acetylation Myelin sheath Cattle Protein Multimerization HeLa Cells |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep40594 |
| Popis: | Tubulin Polymerization Promoting Protein (TPPP/p25) is a brain-specific disordered protein that modulates the dynamics and stability of the microtubule network by its assembly promoting, cross-linking and acetylation enhancing activities. In normal brain it is expressed primarily in differentiated oligodendrocytes; however, at pathological conditions it is enriched in inclusions of both neurons and oligodendrocytes characteristic for Parkinson’s disease and multiple system atrophy, respectively. The objective of this paper is to highlight a critical point of a recently published Skoufias’s paper in which the crucial role of the microtubules in TPPP/p25 dimerization leading to microtubule bundling was suggested. However, our previous and present data provide evidence for the microtubule-independent dimerization of TPPP/p25 and its stabilization by disulphide bridges. In addition, our bimolecular fluorescence complementation experiments revealed the dimerization ability of both the full length and the terminal-free (CORE) TPPP/p25 forms, however, while TPPP/p25 aligned along the bundled microtubule network, the associated CORE segments distributed mostly homogeneously within the cytosol. Now, we identified a molecular model from the possible ones suggested in the Skoufias’s paper that could be responsible for stabilization of the microtubule network in the course of the oligodendrocyte differentiation, consequently in the constitution of the myelin sheath. |
| Databáze: | OpenAIRE |
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