Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis
| Autor: | Jacob P. Thyssen, Peter Brøgger, Stine Simonsen, Sanja Kezic, Lone Skov |
|---|---|
| Přispěvatelé: | Public and occupational health, AII - Infectious diseases, APH - Personalized Medicine, APH - Societal Participation & Health |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Treatment response Pathology medicine.medical_specialty Biopsy medicine.medical_treatment Immunology Mometasone furoate Dermatology Dermatitis Atopic Young Adult Disease severity medicine Stratum corneum Humans RNA Messenger Atopic dermatitis integumentary system business.industry Skin inflammation Middle Aged medicine.disease Cytokine medicine.anatomical_structure Topical corticosteroid Mrna level Case-Control Studies Cytokines Female Epidermis business Biomarkers medicine.drug |
| Zdroj: | Dermatology (Basel, Switzerland), 237(6), 940-945. S. Karger AG |
| ISSN: | 1421-9832 1018-8665 |
| Popis: | Skin biomarkers for disease severity and treatment response in atopic dermatitis (AD) are needed. Biopsies cause scarring and tape stripping represents an alternative minimally invasive method for stratum corneum sampling. In this study, we examined the gene expression of cytokines in skin biopsies and cytokines in stratum corneum tape strips collected from adults with AD. We collected punch biopsies and tape strips from healthy controls (n = 6) and subjects with AD (n = 12) at baseline and after 2 weeks of topical treatment with mometasone furoate 0.1% cream. We found that IFN-γ, IL-13, and IL-10 mRNA (biopsies) and IL-1β protein expression levels (tape strips) were significantly increased in lesional AD skin compared to healthy control skin. Treatment with topical corticosteroid led to a significant decrease in mRNA levels for IL-13 and IL-4R but no significant differences in cytokine protein levels measured in tape strips. Finally, we found no significant correlations between cytokine levels in tape strips and mRNA levels in skin biopsies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|