Egg capsule secretion in invertebrates: a new ovarian regulatory peptide identified by mass spectrometry comparative screening in Sepia officinalis
| Autor: | Joël Henry, Jean Gagnon, Benoit Bernay |
|---|---|
| Přispěvatelé: | Physiologie et Ecophysiologie des Mollusques Marins (PE2M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Transmission et pathogénèse des maladies à prions (TPMP), Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Laboratoire Adaptation et pathogénie des micro-organismes [Grenoble] (LAPM), Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Physiologie et Ecophysiologie des Mollusques Marins ( PE2M ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ) -Centre National de la Recherche Scientifique ( CNRS ), Transmission et pathogénèse des maladies à prions ( TPMP ), Université Joseph Fourier - Grenoble 1 ( UJF ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Adaptation et pathogénie des micro-organismes [Grenoble] ( LAPM ), Institut de biologie structurale ( IBS - UMR 5075 ), Université Joseph Fourier - Grenoble 1 ( UJF ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Grenoble Alpes ( UGA ), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Gagnon, Jean |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
0106 biological sciences
MESH : Molecular Sequence Data Oviposition Peptide MESH: Amino Acid Sequence 01 natural sciences Biochemistry Mass Spectrometry MESH : Oviposition MESH: Ovum MESH : Ovum MESH : Female MESH: Animals Sepia MESH: Oviposition MESH : Seawater chemistry.chemical_classification 0303 health sciences MESH : Peptides MESH: Peptides MESH : Amino Acid Sequence MESH : Spectrometry Mass Electrospray Ionization MESH: Mollusca Cell biology medicine.anatomical_structure Oviduct Female MESH : Mollusca MESH: Ovary medicine.medical_specialty Spectrometry Mass Electrospray Ionization Molecular Sequence Data Biophysics Ovary Biology MESH: Spectrometry Mass Electrospray Ionization MESH: Oocytes 03 medical and health sciences MESH : Mass Spectrometry MESH: Invertebrates Internal medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology medicine Animals Secretion Seawater [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Amino Acid Sequence Molecular Biology [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biology 030304 developmental biology Ovum MESH : Invertebrates MESH : Ovary MESH: Mass Spectrometry MESH: Molecular Sequence Data 010604 marine biology & hydrobiology MESH : Oocytes Capsule MESH: Seawater Cell Biology Oocyte Invertebrates Endocrinology chemistry Mollusca Oocytes Vitellogenesis MESH : Animals Peptides MESH: Female |
| Zdroj: | Biochemical and Biophysical Research Communications Biochemical and Biophysical Research Communications, Elsevier, 2004, 314 (1), pp.215-22 Biochemical and Biophysical Research Communications, 2004, 314 (1), pp.215-22 |
| ISSN: | 0006-291X 1090-2104 |
| Popis: | Mass spectrometry comparative screening was used to identify ovarian regulatory peptides involved in the successive steps of egg-laying in the cuttlefish Sepia officinalis. The peptide content of full-grown oocytes (FGO) was compared with that of oocyte-conditioned medium, which resulted in the detection of peptides that were present in both samples. These peptides, which are suspected of being released by the oocyte in the genital tract, were submitted to a structural analysis. This strategy led to the characterization of a new ovarian regulatory peptide (EISLDKD) able to inhibit the contractions of the whole female genital tract and of the main nidamental glands (MNG). As EISLDKD appeared to be the first regulatory peptide directly involved, at physiological concentrations, in the secretion of the egg capsule by the main nidamental glands, it was named SepCRP for Sepia Capsule Releasing Peptide. Mass spectrometry analysis clearly demonstrated that SepCRP was expressed during vitellogenesis by the ovarian follicles and released by the FGO in the lumen of the female genital tract. In association with the ovarian 5-HT, SepCRP would be responsible for the storage of FGO to avoid the spawning of unfertilized oocytes before mating. Released by the distal oviduct in the mantle cavity, SepCRP probably in association with a cocktail of ovarian regulatory factors targets the MNG to regulate the egg capsule secretion. Thus, the ovary appeared to be one of the main sources of regulatory peptides involved in the successive steps of egg-laying in the cephalopod mollusk S. officinalis. |
| Databáze: | OpenAIRE |
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