Nicotinic Acetylcholine Receptors Mediate the Suppressive Effect of an Injection of Diluted Bee Venom into the GV3 Acupoint on Oxaliplatin-Induced Neuropathic Cold Allodynia in Rats
Autor: | Sun Kwang Kim, Insoo Yoon, Hyunsu Bae, Min Joon Kim, Dong Xing Li, Heera Yoon |
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Rok vydání: | 2015 |
Předmět: |
Male
Organoplatinum Compounds medicine.drug_class Apitherapy Pharmaceutical Science Antineoplastic Agents Muscarinic Antagonists Nicotinic Antagonists Pharmacology Receptors Nicotinic Injections Rats Sprague-Dawley chemistry.chemical_compound Muscarinic acetylcholine receptor Mecamylamine medicine Animals Nicotinic Antagonist Methyllycaconitine business.industry Antagonist General Medicine Receptor antagonist Cold Temperature Oxaliplatin Bee Venoms Nicotinic agonist Allodynia chemistry Spinal Cord Hyperalgesia Anesthesia Neuralgia medicine.symptom business Acupuncture Points medicine.drug |
Zdroj: | Biologicalpharmaceutical bulletin. 38(5) |
ISSN: | 1347-5215 |
Popis: | Oxaliplatin, a platinum-based chemotherapy drug, often induces acute neuropathic pain, especially cold allodynia, even after a single administration. Subcutaneous injection of diluted bee venom (BV) into acupoints has been used to treat various pain symptoms in traditional oriental medicine. Although we previously demonstrated the suppressive effect of BV injection on oxaliplatin-induced cold allodynia in rats, its neurochemical mechanism remained unclear. This study investigates whether and how the cholinergic system mediates the relieving effect of BV injection on cold allodynia in oxaliplatin-administered rats. The behavioral signs of cold allodynia induced by an oxaliplatin administration (6 mg/kg, intraperitoneally (i.p.)) were evaluated by a tail immersion test in cold water (4°C). BV (0.25 mg/kg, subcutaneously (s.c.)) injection into the Yaoyangguan acupoint, located between the spinous processes of the fourth and fifth lumbar vertebrae, significantly alleviated the cold allodynia. This relieving effect of BV injection on oxaliplatin-induced cold allodynia was blocked by a pretreatment with mecamylamine (a non-selective nicotinic receptor antagonist, 2 mg/kg, i.p.), but not by atropine (a non-selective muscarinic receptor antagonist, 1 mg/kg, i.p.). Further, dihydro-β-erythroidinehydrobromide (DHβE, an α4β2 nicotinic antagonist, 5 mg/kg, i.p.) prevented the anti-allodynic effect of BV, whereas methyllycaconitine (an α7 nicotinic antagonist, 6 mg/kg, i.p.) did not. Finally, intrathecal administration of DHβE (10 nM) blocked the BV-induced anti-allodynic effect. These results suggest that nicotinic acetylcholine receptors, especially spinal α4β2 receptors, but not muscarinic receptors, mediate the suppressive effect of BV injection on oxaliplatin-induced acute cold allodynia in rats. |
Databáze: | OpenAIRE |
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