Treatment Efficiency of Different Routes of Bone Marrow-Derived Mesenchymal Stem Cell Injection in Rat Liver Fibrosis Model

Autor: Amany Osama, Naglaa K. Idriss, Dina Sabry, Hayam G. Sayyed
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Liver Cirrhosis
Male
0301 basic medicine
Pathology
medicine.medical_specialty
Physiology
medicine.medical_treatment
Liver fibrosis
Connective tissue
Bone Marrow Cells
Mesenchymal Stem Cell Transplantation
lcsh:Physiology
Transforming Growth Factor beta1
lcsh:Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Fibrosis
medicine
Animals
lcsh:QD415-436
Carbon Tetrachloride
Transplantation route
Keratin-18
lcsh:QP1-981
Hepatocyte Growth Factor
business.industry
Growth factor
Mesenchymal stem cell
Connective Tissue Growth Factor
Mesenchymal Stem Cells
medicine.disease
Actins
Rats
Vascular endothelial growth factor
Transplantation
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
Liver
chemistry
Administration
Intravenous
Liver function
Bone marrow
business
Spleen
BM-MSCs
Zdroj: Cellular Physiology and Biochemistry, Vol 48, Iss 5, Pp 2161-2171 (2018)
ISSN: 1421-9778
1015-8987
Popis: Background/Aims: The most appropriate route for bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation in the management of liver fibrosis remains controversial. This study investigated the therapeutic efficacy of intravenous and intrasplenic BM-MSC transplantation on carbon tetrachloride (CCl4)-induced rat liver fibrosis. Methods: Fifty rats were divided into 5 groups (n = 10 rats per group): healthy control group, CCl4 group, CCl4/ recovery group, CCl4/BM-MSC intravenous group, and CCl4/BM-MSC intrasplenic group. BM-MSCs were isolated, labeled with green fluorescent protein (GFP), and injected into fibrotic rats either intravenously or intrasplenically. Gene expression of interleukins (IL-1β and IL-6), interferon (INF)-γ, hepatic growth factor, and the hepatocyte-specific marker cytokeratin 18 was estimated by quantitative real-time reverse transcription-polymerase chain reaction. Vascular endothelial growth factor and connective tissue growth factor was detected by western blot analysis and enzyme-linked immunosorbent assay, respectively. At 2 weeks after intravenous and intrasplenic BM-MSC injections, GFP-positive cells were detected in liver tissue. Results: Both routes achieved a similar enhancement of liver function, which was confirmed by histopathological examination. The intravenous route was more effective than the intrasplenic route in reducing gene expression levels of IL-1β, IL-6, and INF-γ. However, fibrotic changes were still observed in the recovery group. Conclusion: Intravenous BM-MSC injection was an efficient and appropriate route for BM-MSC transplantation for the management of liver fibrosis.
Databáze: OpenAIRE
Externí odkaz: