Reclassification of Chromobacterium violaceum ATCC 31532 and its quorum biosensor mutant CV026 to Chromobacterium subtsugae
| Autor: | Alisha Harrison, Scott D. Soby |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Gram-negative bacteria
lcsh:Biotechnology Mutant lcsh:QR1-502 Biophysics Molecular phylogeny Applied Microbiology and Biotechnology lcsh:Microbiology Microbiology 03 medical and health sciences chemistry.chemical_compound Chromobacterium lcsh:TP248.13-248.65 030304 developmental biology 0303 health sciences biology Chromobacterium subtsugae 030306 microbiology N-Acyl-homoserine lactone biology.organism_classification Quorum sensing N-Acyl homoserine lactone chemistry Original Article Chromobacterium violaceum Bacteria Biosensor |
| Zdroj: | AMB Express AMB Express, Vol 10, Iss 1, Pp 1-7 (2020) |
| ISSN: | 2191-0855 |
| Popis: | The precipitous drop in the cost of genomic sequencing and the concomitant availability of computational methods for comparing genome-level data has made the accurate taxonomic placement of bacteria affordable and relatively rapid. Inaccurate taxonomic placement of bacteria has serious implications in clinical, environmental, and regulatory microbiology, but it can also adversely affect interpretation of research results. The quorum biosensor strain CV026 was derived from an isolate of Chromobacterium that was labeled as C. violaceum ATCC 31532, and is catalogued by the ATCC under that species name. Nearly 200 papers have been published that use CV026 as an indicator for quorum sensing activity in many Gram negative bacteria, but the inability of C. violaceum strains to complement the quorum sensing mutation in CV026 has called the taxonomic placement of the parent strain into question. We used molecular phylogeny and a large number of metabolic and phenotypic characters to demonstrate that Chromobacterium strain ATCC 31532 is a member of species Chromobacterium subtsugae. |
| Databáze: | OpenAIRE |
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