The Crystal Structure of Mycobacterium tuberculosisAlkylhydroperoxidase AhpD, a Potential Target for Antitubercular Drug Design
| Autor: | Paul R. Ortiz de Montellano, Clinton R. Nishida, Snezana Djordjevic, Patrick J. Hillas, Christine M. Nunn |
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| Rok vydání: | 2002 |
| Předmět: |
Models
Molecular Protein family Stereochemistry Protein subunit Antitubercular Agents Trimer Biology Crystallography X-Ray Biochemistry Mycobacterium tuberculosis chemistry.chemical_compound Oxidoreductase Catalytic Domain medicine Histidine Cysteine Selenomethionine Molecular Biology chemistry.chemical_classification Binding Sites Isoniazid Peroxiredoxins Cell Biology biology.organism_classification Models Chemical Peroxidases chemistry Sulfenic acid Dimerization Protein Binding medicine.drug |
| Zdroj: | Journal of Biological Chemistry. 277:20033-20040 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m200864200 |
| Popis: | The resistance of Mycobacterium tuberculosis to isoniazid is commonly linked to inactivation of a catalase-peroxidase, KatG, that converts isoniazid to its biologically active form. Loss of KatG is associated with elevated expression of the alkylhydroperoxidases AhpC and AhpD. AhpD has no sequence identity with AhpC or other proteins but has alkylhydroperoxidase activity and possibly additional physiological activities. The alkylhydroperoxidase activity, in the absence of KatG, provides an important antioxidant defense. We have determined the M. tuberculosis AhpD structure to a resolution of 1.9 A. The protein is a trimer in a symmetrical cloverleaf arrangement. Each subunit exhibits a new all-helical protein fold in which the two catalytic sulfhydryl groups, Cys-130 and Cys-133, are located near a central cavity in the trimer. The structure supports a mechanism for the alkylhydroperoxidase activity in which Cys-133 is deprotonated by a distant glutamic acid via the relay action of His-137 and a water molecule. The cysteine then reacts with the peroxide to give a sulfenic acid that subsequently forms a disulfide bond with Cys-130. The crystal structure of AhpD identifies a new protein fold relevant to members of this protein family in other organisms. The structural details constitute a potential platform for the design of inhibitors of potential utility as antitubercular agents and suggest that AhpD may have disulfide exchange properties of importance in other areas of M. tuberculosis biology. |
| Databáze: | OpenAIRE |
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