| Autor: |
Takahisa Hirai, Shin-ichiro Masunaga, Akira Sato, Hiroaki Fujimori, Zhaxybay Zhumadilov, Hiroyuki Nakamura, Hiroki Tanaka, Diaz Baiseitov, Yoshinori Sakurai, Kulzhan Berikkhanova, Minoru Suzuki, Sota Kikuhara, Shoji Imamichi, Mitsuko Masutani, Koji Ono, Jun Itami, Yasufumi Murakami, Tasuku Itoh, Yoshio Imahori, Soichiro Saito |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine. 106 |
| ISSN: |
1872-9800 |
| Popis: |
To understand the mechanism of cell death induced by boron neutron capture reaction (BNCR), we performed proteome analyses of human squamous tumor SAS cells after BNCR. Cells were irradiated with thermal neutron beam at KUR after incubation under boronophenylalanine (BPA)(+) and BPA(-) conditions. BNCR mainly induced typical apoptosis in SAS cells 24h post-irradiation. Proteomic analysis in SAS cells suggested that proteins functioning in endoplasmic reticulum, DNA repair, and RNA processing showed dynamic changes at early phase after BNCR and could be involved in the regulation of cellular response to BNCR. We found that the BNCR induces fragments of endoplasmic reticulum-localized lymphoid-restricted protein (LRMP). The fragmentation of LRMP was also observed in the rat tumor graft model 20 hours after BNCT treatment carried out at the National Nuclear Center of the Republic of Kazakhstan. These data suggest that dynamic changes of LRMP could be involved during cellular response to BNCR. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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