Key Electrophysiological, Molecular, and Metabolic Signatures of Sleep and Wakefulness Revealed in Primary Cortical Cultures
| Autor: | Paul Franken, Johan Auwerx, Thomas Curie, Riekelt H. Houtkooper, Mehdi Tafti, Cyril Mikhail, Sylvain Pradervand, Valérie Hinard |
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| Přispěvatelé: | Laboratory Genetic Metabolic Diseases |
| Rok vydání: | 2012 |
| Předmět: |
Male
Action Potentials/physiology Animals Cells Cultured Cerebral Cortex/chemistry Cerebral Cortex/metabolism Electrophysiological Phenomena/physiology Female Mice Mice Inbred C57BL Sleep/physiology Sleep Deprivation/metabolism Wakefulness/physiology Action Potentials Stimulation AMPA receptor Biology Transcriptome 03 medical and health sciences 0302 clinical medicine medicine Wakefulness Neuroscience of sleep 030304 developmental biology Cerebral Cortex 0303 health sciences General Neuroscience Articles Sleep in non-human animals Electrophysiological Phenomena Electrophysiology Sleep deprivation Sleep Deprivation medicine.symptom Sleep Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neuroscience, vol. 32, no. 36, pp. 12506-12517 Journal of neuroscience, 32(36), 12506-12517. Society for Neuroscience The Journal of neuroscience : the official journal of the Society for Neuroscience |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.2306-12.2012 |
| Popis: | Although sleep is defined as a behavioral state, at the cortical level sleep has local and use-dependent features suggesting that it is a property of neuronal assemblies requiring sleep in function of the activation experienced during prior wakefulness. Here we show that mature cortical cultured neurons display a default state characterized by synchronized burst–pause firing activity reminiscent of sleep. This default sleep-like state can be changed to transient tonic firing reminiscent of wakefulness when cultures are stimulated with a mixture of waking neurotransmitters and spontaneously returns to sleep-like state. In addition to electrophysiological similarities, the transcriptome of stimulated cultures strikingly resembles the cortical transcriptome of sleep-deprived mice, and plastic changes as reflected by AMPA receptors phosphorylation are also similar. We used ourin vitromodel and sleep-deprived animals to map the metabolic pathways activated by waking. Only a few metabolic pathways were identified, including glycolysis, aminoacid, and lipids. Unexpectedly large increases in lysolipids were found bothin vivoafter sleep deprivation andin vitroafter stimulation, strongly suggesting that sleep might play a major role in reestablishing the neuronal membrane homeostasis. With ourin vitromodel, the cellular and molecular consequences of sleep and wakefulness can now be investigated in a dish. |
| Databáze: | OpenAIRE |
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