Effects of exercise training combined with insulin treatment on cardiac NOS1 signaling pathways in type 1 diabetic rats

Autor: François Carré, M. S. Zguira, Solène Le Douairon Lahaye, Arlette Gratas-Delamarche, Françoise Rannou Bekono, Amélie Rébillard, Ludivine Malardé, Bernard Saïag
Přispěvatelé: Laboratoire Mouvement Sport Santé (M2S), École normale supérieure - Cachan (ENS Cachan)-Université de Rennes (UR)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), École normale supérieure - Cachan (ENS Cachan)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Université de Brest (UBO)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Blood Glucose
Male
MESH: Signal Transduction
medicine.medical_treatment
MESH: Organ Size
Clinical Biochemistry
Blood Pressure
MESH: Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type I
MESH: Physical Conditioning
Animal
030204 cardiovascular system & hematology
Ryanodine receptor 2
MESH: Ryanodine Receptor Calcium Release Channel
0302 clinical medicine
Diastole
Heart Rate
MESH: Diabetes Mellitus
Experimental
Insulin
MESH: Animals
MESH: Heart Rate
0303 health sciences
MESH: Muscle
Skeletal
biology
MESH: Diastole
Organ Size
General Medicine
MESH: Blood Pressure
MESH: Systole
3. Good health
Nitric oxide synthase
Type 1 diabetes
cardiovascular system
MESH: Nitric Oxide Synthase
[SDV.IB]Life Sciences [q-bio]/Bioengineering
MESH: Citrate (si)-Synthase
MESH: Diabetes Mellitus
Type 1
Signal Transduction
Cardiac function curve
medicine.medical_specialty
MESH: Myocardium
MESH: Rats
Systole
Heart Ventricles
Citrate (si)-Synthase
MESH: Insulin
In Vitro Techniques
Diabetes Mellitus
Experimental
03 medical and health sciences
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
Physical Conditioning
Animal
Diabetes mellitus
Internal medicine
medicine
Animals
Rats
Wistar
Muscle
Skeletal
Molecular Biology
Exercise
030304 developmental biology
business.industry
Myocardium
Body Weight
Cardiac function
Ryanodine Receptor Calcium Release Channel
Cell Biology
MESH: Rats
Wistar
medicine.disease
MESH: Male
Rats
MESH: Body Weight
Diabetes Mellitus
Type 1
Endocrinology
Basal (medicine)
Receptors
Adrenergic
beta-3
biology.protein
MESH: Blood Glucose
MESH: Heart Ventricles
business
MESH: Receptors
Adrenergic
beta-3
Zdroj: Molecular and Cellular Biochemistry
Molecular and Cellular Biochemistry, 2011, 347 (1-2), pp.53-62. ⟨10.1007/s11010-010-0611-6⟩
Molecular and Cellular Biochemistry, Springer Verlag, 2011, 347 (1-2), pp.53-62. ⟨10.1007/s11010-010-0611-6⟩
ISSN: 0300-8177
1573-4919
DOI: 10.1007/s11010-010-0611-6⟩
Popis: International audience; This study examined the effects of a dual treatment combining insulin treatment and exercise training on basal cardiac function and signaling pathways involving β3-AR, NOS1, and RyR2 in type 1 diabetic rats. Male Wistar rats were assigned into a diabetic group receiving no treatment (D), an insulin-treated diabetic (Ins), a trained diabetic (TD), and a trained insulin-treated diabetic (TIns) group. Control group (C) was included in order to confirm the deleterious effects of diabetes. Insulin treatment and/or treadmill exercise training were conducted for 8 weeks. Basal cardiac function was evaluated by Langendorff technique. Cardiac protein expression of β3-AR, NOS1, and RyR2 was assessed using Western blots. Diabetes induced a decrease of both basal diastolic and systolic (±dP/dt) cardiac function (P < 0.05). Moreover, diabetes was associated with an increase of β3-AR and NOS1 and a decrease of RyR2 expression (P < 0.05). Although combined treatment was not able to normalize -dP/dt, it succeeded to normalize +dP/dt of diabetic rats. Combined treatment led to an overexpression of RyR2. Effects of this combined treatment on +dP/dt and RyR2 were greater than the effects of insulin and exercise training, applied solely. Treatments, applied solely or in combination, resulted in a complete normalization of β3-AR and in a down-regulation of NOS1 because this protein expression in all treated diabetic rats became lower than control values (P < 0.01). Our study shows that unlike single treatments, dual treatment combining insulin treatment and exercise training was able to normalize basal systolic function of diabetic rats by a specific regulation of β3-AR-NOS1-RyR2 signaling pathways.
Databáze: OpenAIRE
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