Diagnostic and prognostic value of tissue and circulating levels of Ephrin-A2 in prostate cancer
| Autor: | Zhiyuan Wu, Xinju Zhang, Ping He, Xiao Xu, Shibao Li, Hua Wang, Qunfeng Zhang, Zhihua Kang, Yanqun Cai, Yuming Chen, Ming Guan, Tingting Hu |
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| Rok vydání: | 2015 |
| Předmět: |
Male
0301 basic medicine Oncology PCA3 medicine.medical_specialty urologic and male genital diseases 03 medical and health sciences Prostate cancer 0302 clinical medicine DU145 Prostate Internal medicine LNCaP Biomarkers Tumor Humans Medicine Aged Neoplasm Staging Aged 80 and over biology business.industry Ephrin-A2 Prostatic Neoplasms General Medicine Neoplastic Cells Circulating Prognosis medicine.disease Gene Expression Regulation Neoplastic Ki-67 Antigen 030104 developmental biology medicine.anatomical_structure Tumor progression 030220 oncology & carcinogenesis Ki-67 biology.protein Ephrin A2 Neoplasm Grading business |
| Zdroj: | Tumor Biology. 37:5365-5374 |
| ISSN: | 1423-0380 1010-4283 |
| DOI: | 10.1007/s13277-015-4398-7 |
| Popis: | Ephrin-A2, a member of the Eph/ephrin family, is associated with tumorigenesis and tumor progression. This study aimed to assess the diagnostic and prognostic value of both serum and tissue levels of Ephrin-A2 in prostate cancer (PCa) management. One hundred and forty-five frozen prostate tissues, 55 paraffin-embedded prostate tissues, 88 serum samples, and seven prostate cell lines (RWPE-1, LNCaP, LNCaP-LN3, PC-3, PC-3M, PC-3M-LN4, and DU145) were examined via quantitative reverse transcription-PCR (qRT-PCR), immunohistochemistry, enzyme-linked immunosorbent assay, and western blotting. Induced Ephrin-A2 messenger RNA (mRNA) or protein expression was detected in 8.6 % (5/58) benign prostatic hyperplasia (BPH), 59.8 % (52/87) PCa, and five prostate cancer cell lines. Ephrin-A2 immunostaining was present in 6.7 % (1/15) patients with BPHs and 62.5 % (25/40) clinically localized PCa. Accordingly, serum Ephrin-A2 was significantly higher in PCa patients compared to those in the BPH patients and controls (P |
| Databáze: | OpenAIRE |
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