26-Locus Y-STR typing in a Bhutanese population sample
| Autor: | Emma J. Parkin, Thirsa Kraayenbrink, George L. van Driem, Karma Tshering of Gaselô, Peter de Knijff, Mark A. Jobling |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Genetics education.field_of_study Chromosomes Human Y Amelogenin STR multiplex system Population Haplotype Locus (genetics) Biology DNA Fingerprinting Polymerase Chain Reaction Pathology and Forensic Medicine Genetics Population Dental Enamel Proteins Gene Frequency DNA profiling Tandem Repeat Sequences Multiplex polymerase chain reaction Humans Y-STR Multiplex Bhutan education Law |
| Zdroj: | Forensic Science International. 161:1-7 |
| ISSN: | 0379-0738 |
| Popis: | 26 Y chromosome short tandem repeat (STR) loci were amplified in a sample of 856 unrelated males from Bhutan, using two multiplex polymerase chain reaction (PCR) assays. The first multiplex is the Y-STR 20plex described by Butler et al. [J.M. Butler, R. Schoske, P.M. Vallone, M.C. Kline, A.J. Redd, M.F. Hammer, A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers, Forensic Sci. Int. 129 (2002) 10-24], and the second is a novel (but overlapping) 14plex that targets six additional Y-STRs (DYS425, DYS434, DYS435, DYS436, DYS461, DYS462) and also amplifies the amelogenin locus. The 26-loci give a discriminating power of 0.9957, though even at this resolution one haplotype occurs 24 times. We identify novel alleles at five loci and microvariants at a further three, which were characterised by sequencing. Extended (11-locus) haplotypes for these samples have been submitted to the Y-STR Haplotype Reference Database (YHRD). |
| Databáze: | OpenAIRE |
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