Blood content in guinea-pig tissues: correction for the study of drug tissue distribution
| Autor: | Hsiu-Ping Yu, Tien-Chun Chang, Yuh-Wen Chiu, Yu-Zen Shen, Hsin-Hong Lin |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pharmacology
Male medicine.medical_specialty Kidney Lung biology Valproic Acid Guinea Pigs Serum albumin Adipose tissue Spleen Viscera medicine.anatomical_structure Endocrinology Pharmacokinetics Internal medicine Immunology medicine biology.protein Distribution (pharmacology) Animals Female Tissue Distribution Bovine serum albumin Serum Albumin Radio-Iodinated |
| Zdroj: | Pharmacological research. 23(4) |
| ISSN: | 1043-6618 |
| Popis: | The blood content in different organs or tissues of guinea-pigs was determined by means of [125I]bovine serum albumin. The blood distribution expressed as percentage of total body blood for various organs or tissues ranged from 0.35 (adipose) to 17.5 (muscle)%. The blood content in tissue preparations expressed as the blood background correction factor, F (ml blood/g wet tissue) depended on the extent of bleeding during experiment; that factor can cause a considerable difference in the results of a tissue distribution study of a drug. Blood content was high in the lung (0.36 ml/g), heart, liver, kidney and spleen, but low in adipose and brain tissues (0.021 ml/g). The distribution of valproic acid in various tissues of guinea-pigs after intravenous injection was determined from the homogenates of isolated organs. The results showed that blood contamination can greatly alter the data of tissue distribution of a drug to as much as 25%. This study proposed a maximum (non-bled) and a minimum (extreme-bled) blood background correction factor for respective tissues of guinea-pigs, as well as equations for correcting the blood contamination in tissue during the drug distribution study. The results suggest that to obtain an exact analysis of drug concentrations in specific tissues, a correction for the blood of that specific tissue is necessary. |
| Databáze: | OpenAIRE |
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