Phytochemical study guided by the myorelaxant activity of the crude extract, fractions and constituent from stem bark of Hymenaea courbaril L
| Autor: | Mary Anne Medeiros Bandeira, Francisco J.B. Lima, Nirla Rodrigues Romero, Roberto Wagner da Silva Góis, Gabrieli da Penha Bezerra, Pedro Jorge Caldas Magalhães, Teresinha S. Brito, Gilvandete M. P. Santiago |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Antioxidant DPPH Muscle Relaxation medicine.medical_treatment Flavonoid Anti-Inflammatory Agents Ethyl acetate Chemical Fractionation In Vitro Techniques Antioxidants chemistry.chemical_compound Drug Discovery Botany Respiratory Hypersensitivity medicine Animals Hymenaea Rats Wistar Hymenaea courbaril Pharmacology chemistry.chemical_classification Dose-Response Relationship Drug Plant Stems Traditional medicine biology Plant Extracts biology.organism_classification Rats Trachea chemistry Phytochemical Ethnopharmacology Plant Bark Medicine Traditional Astilbin Brazil |
| Zdroj: | Journal of Ethnopharmacology. 149:62-69 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2013.05.052 |
| Popis: | Ethnopharmacological relevance Hymenaea courbaril L. (Caesalpinoideae) is used in Brazilian folk medicine to treat anemia, kidney problems, sore throat and other dysfunctions of the respiratory system, such as bronchitis and asthma, although such properties are yet to be scientifically validated. Aim of the study In order to give a scientific basis to support the traditional use of Hymenaea courbaril, this study was designed to evaluate antioxidant, myorelaxant and anti-inflammatory properties of the ethanol extract from stem bark and its fractions. The myorelaxant effect of astilbin, a flavonoid isolated from the bioactive ethyl acetate fraction (EAF), has also been evaluated. Material and methods In the present study ethanol extract from stem bark (EEHC) and fractions were analyzed using bioassay-guided fractionation. The following activities were investigated: antioxidant by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, myorelaxant on rat tracheal smooth muscle, and anti-inflammatory using ovalbumin-induced leukocytosis and airway hyperresponsiveness in rats. Results The results of the present investigation show that the whole extract of Hymenaea courbaril and some of its fractions strongly scavenged DPPH radical. The extract showed myorelaxant activity on rat trachea, being EAF its highest efficient fraction. Bio-guided study allowed the isolation of astilbin, a well-known flavonoid. The activity induced by this compound indicates that it may be partly responsible for the myorelaxant effect of EAF. EAF reduced contractions that depended on divalent cation inflow through voltage-operated Ca2+ channels (VOCCs) or receptor-operated Ca2+ channels (ROCCs), but it was more potent to inhibit VOCC- than ROCC-dependent contraction induced by Ca2+ addition in ACh-enriched Ca2+-free medium. Oral pretreatment of antigen-challenged animals with EAF prevented airway hyperresponsiveness on KCl-induced contraction and reduced the number of total white cells, particularly eosinophils and neutrophils in bronchoalveolar lavage. Conclusions This study provided scientific basis that Hymenaea courbaril presents potential antioxidant, myorelaxant and anti-inflammatory actions, which support its use in folk medicine to treat inflammatory airway diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect