Urine 5-Eicosatetraenoic Acids as Diagnostic Markers for Obstructive Sleep Apnea
| Autor: | Jong Wan Park, Hyun Woo Shin, Daeho So, Joo Youn Cho, Ji Young Kim, Sung Wan Kim, Il Hee Hong, Seok Hyun Cho, Chae Seo Rhee, K.H. Cho |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Physiology medicine.medical_treatment Clinical Biochemistry Polysomnography Urine RM1-950 Biochemistry Gastroenterology Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Continuous positive airway pressure Molecular Biology obstructive sleep apnea chemistry.chemical_classification Creatinine medicine.diagnostic_test business.industry hypoxia Glutathione peroxidase Intermittent hypoxia Cell Biology Hypoxia (medical) 5-HETE medicine.disease metabolomics respiratory tract diseases Obstructive sleep apnea 5-oxoETE 030104 developmental biology chemistry urine biomarker Therapeutics. Pharmacology medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Antioxidants, Vol 10, Iss 1242, p 1242 (2021) Antioxidants Volume 10 Issue 8 |
| ISSN: | 2076-3921 |
| Popis: | Early detection of obstructive sleep apnea (OSA) is needed to reduce cardiovascular sequelae and mortality. Full-night polysomnography has been used for diagnosing OSA, but it is too expensive and inconvenient for patients to handle. Metabolome-wide analyses were performed to find and validate surrogate markers for OSA. We further investigated the mechanism underlying hypoxic induction of the markers in human cells and mice. Arachidonic acid derivatives 5-HETE and 5-oxoETE were detected in urine samples. The levels (mean ± SD, ng per mg creatinine) of 5-HETE and 5-oxoETE were 56.4 ± 26.2 and 46.9 ± 18.4 in OSA patients, respectively, which were significantly higher than those in controls (22.5 ± 4.6 and 18.7 ± 3.6). Both levels correlated with the apnea-hypopnea index and the lowest oxygen saturation on polysomnography. After the treatment with the continuous positive airway pressure, the metabolite levels were significantly reduced compared with those before the treatment. In human mononuclear cells subjected to intermittent hypoxia, 5-HETE and 5-oxoETE productions were induced by hypoxia-inducible factor 1 and glutathione peroxidase. When mice were exposed to intermittent hypoxia, 5-HETE and 5-oxoETE were excreted more in urine. They were identified and verified as new OSA markers reflecting hypoxic stress. The OSA markers could be used for OSA diagnosis and therapeutic evaluation. |
| Databáze: | OpenAIRE |
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