The role of aminopeptidase N in Met-enkephalin modulated superoxide anion release
| Autor: | Marija Abramić, Ivna Svoboda-Beusen, Tihomir Balog, Ivo Hršak, Tanja Marotti |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Agonist Met-enkephalin medicine.medical_specialty animal structures Neutrophils medicine.drug_class Enkephalin Methionine Neuropeptide CD13 Antigens chemistry.chemical_compound Leucine Superoxides Internal medicine medicine Humans Protease Inhibitors Enzyme Inhibitors Pharmacology Analgesics Chemistry Superoxide Proteolytic enzymes Enkephalins Enkephalin Ala(2)-MePhe(4)-Gly(5) Respiratory burst Analgesics Opioid Enzyme Activation Endocrinology Mechanism of action Liberation Female the role of aminopeptidase N in Met-enkephalin modulated superoxide anion release medicine.symptom Enkephalin D-Penicillamine (2 5) Oligopeptides hormones hormone substitutes and hormone antagonists |
| Popis: | We have previously shown that methionine-enkephalin (MENK) alters in dose-dependent fashion the capacity of human neutrophils to produce superoxide anion. The response of neutrophils from different donors was diverse and this effect could be due to variable activity of proteolytic enzymes involved in the degradation of the neuropeptide. In this study, we have demonstrated a highly individual aminopeptidase N (APN) activity of neutrophils from different donors. Preincubation of neutrophils with MENK, but not with the synthetic agonist of the μ (DAGO) or the δ (DPDPE) opioid receptor, down-regulated the APN activity. This was paralleled by a loss in cell surface expression of APN at physiological (10 −10 M) concentrations of MENK. The level of APN activity from different donors correlated with the effect of MENK on superoxide anion release. Neutrophils with low APN activity, if preincubated with MENK, released reduced amounts of superoxide anion. In contrast, neutrophils with high APN activity released increased amounts of superoxide anion after preincubation with MENK. Thus, the highly individual APN activity on the surface of neutrophils from different donors seems to be altered by MENK and to be related to the respiratory burst. |
| Databáze: | OpenAIRE |
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