Comparative Analyses of Cytochrome P450s and Those Associated with Secondary Metabolism in Bacillus Species

Autor: Jae-Hyuk Yu, Abidemi Paul Kappo, Wanping Chen, Khajamohiddin Syed, Mathula Lancelot Ngwenya, David R. Nelson, Bongumusa Comfort Mthethwa, Rajshekhar Karpoormath, Puleng Rosinah Syed
Rok vydání: 2018
Předmět:
cytochrome P450 monooxygenase
0301 basic medicine
Cytochrome
Secondary Metabolism
Bacillus
lcsh:Chemistry
Cytochrome P-450 Enzyme System
Antibiotics
polycyclic compounds
Mycobacterium
lcsh:QH301-705.5
Phylogeny
Spectroscopy
biology
secondary metabolites
P450 profiling
General Medicine
respiratory system
biosynthetic gene clusters
Computer Science Applications
Biochemistry
Multigene Family
medicine.drug
comparative analysis
In silico
Secondary metabolite
digestive system
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Species Specificity
medicine
Physical and Theoretical Chemistry
Secondary metabolism
Molecular Biology
030102 biochemistry & molecular biology
Organic Chemistry
Cytochrome P450
Monooxygenase
P450 diversity percentage
biology.organism_classification
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
biology.protein
human activities
Bacteria
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 19, Iss 11, p 3623 (2018)
Volume 19
Issue 11
ISSN: 1422-0067
DOI: 10.3390/ijms19113623
Popis: Cytochrome P450 monooxygenases (CYPs/P450s) are among the most catalytically-diverse enzymes, capable of performing enzymatic reactions with chemo-, regio-, and stereo-selectivity. Our understanding of P450s&rsquo
role in secondary metabolite biosynthesis is becoming broader. Among bacteria, Bacillus species are known to produce secondary metabolites, and recent studies have revealed the presence of secondary metabolite biosynthetic gene clusters (BGCs) in these species. However, a comprehensive comparative analysis of P450s and P450s involved in the synthesis of secondary metabolites in Bacillus species has not been reported. This study intends to address these two research gaps. In silico analysis of P450s in 128 Bacillus species revealed the presence of 507 P450s that can be grouped into 13 P450 families and 28 subfamilies. No P450 family was found to be conserved in Bacillus species. Bacillus species were found to have lower numbers of P450s, P450 families and subfamilies, and a lower P450 diversity percentage compared to mycobacterial species. This study revealed that a large number of P450s (112 P450s) are part of different secondary metabolite BGCs, and also identified an association between a specific P450 family and secondary metabolite BGCs in Bacillus species. This study opened new vistas for further characterization of secondary metabolite BGCs, especially P450s in Bacillus species.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje