Superantigen-reactive CD4+ T cells are required to stimulate B cells after infection with mouse mammary tumor virus
| Autor: | Hans Acha-Orbea, H R MacDonald, A N Shakhov, Shozo Izui, Leonardo Scarpellino, Werner Held, Gary A. Waanders |
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| Rok vydání: | 1993 |
| Předmět: |
RNA
Messenger/genetics CD4-Positive T-Lymphocytes B-Lymphocytes/ immunology/microbiology Genes Viral viruses ddc:616.07 Lymphocyte Activation Mice Cell–cell interaction Antigens Viral/immunology Superantigen Immunology and Allergy Antigens Viral B-Lymphocytes Mice Inbred BALB C biology Animals Antibody Formation B-Lymphocytes/immunology B-Lymphocytes/microbiology Base Sequence CD4-Positive T-Lymphocytes/immunology Gene Expression Regulation Viral Mammary Tumor Virus Mouse/genetics Mammary Tumor Virus Mouse/immunology Mice Nude Minor Lymphocyte Stimulatory Antigens/immunology Molecular Sequence Data Oligodeoxyribonucleotides/chemistry RNA Viral/genetics Tumor Virus Infections/immunology Tumor Virus Infections/microbiology Viral Structural Proteins/genetics Articles medicine.anatomical_structure Oligodeoxyribonucleotides RNA Viral Immunology chemical and pharmacologic phenomena Minor Lymphocyte Stimulatory Antigens Immune system Antigen Mammary Tumor Virus Mouse/genetics/ immunology medicine CD4-Positive T-Lymphocytes/ immunology RNA Messenger B cell Viral Structural Proteins Mouse mammary tumor virus T-cell receptor Minor Lymphocyte Stimulatory Antigens/ immunology Tumor Virus Infections/ immunology/microbiology biology.organism_classification Virology Molecular biology Tumor Virus Infections Mammary Tumor Virus Mouse |
| Zdroj: | Journal of Experimental Medicine, vol. 177, no. 2, pp. 359-366 The Journal of Experimental Medicine Journal of Experimental Medicine, Vol. 177, No 2 (1993) pp. 359-366 |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | Superantigens are defined by their ability to stimulate a large fraction of T cells via interaction with the T cell receptor (TCR) V beta domain. Endogenous superantigens, classically termed minor lymphocyte-stimulating (Mls) antigens, were recently identified as products of open reading frames (ORF) in integrated proviral copies of mouse mammary tumor virus (MMTV). We have described an infectious MMTV homologue of the classical endogenous superantigen Mls-1a (Mtv-7). The ORF molecules of both the endogenous Mtv-7 and the infectious MMTV(SW) interact with T cells expressing the TCR V beta 6, 7, 8.1, and 9 domains. Furthermore, the COOH termini of their ORF molecules, thought to confer TCR specificity, are very similar. Since successful transport of MMTV from the site of infection in the gut to the mammary gland depends on a functional immune system, we were interested in determining the early events after and requirements for MMTV infection. We show that MMTV(SW) infection induces a massive response of V beta 6+ CDC4+ T cells, which interact with the viral ORF. Concomitantly, we observed a B cell response and differentiation that depends on both the presence and stimulation of the superantigen-reactive T cells. Furthermore, we show that B cells are the main target of the initial MMTV infection as judged by the presence of the reverse-transcribed viral genome and ORF transcripts. Thus, we suggest that MMTV infection of B cells leads to ORF-mediated B-T cell interaction, which maintains and possibly amplifies viral infection. |
| Databáze: | OpenAIRE |
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