Rapid initiation of neurite outgrowth onto laminin from explants of adult mouse retina induced by optic nerve crush
| Autor: | Ronald L. Meyer, Jill Miotke |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Neurite
Nerve Crush medicine.medical_treatment Mice Inbred Strains Biology Retinal ganglion Retina Mice chemistry.chemical_compound Developmental Neuroscience Laminin medicine Animals Microscopy Phase-Contrast Polylysine integumentary system Optic Nerve Serum Albumin Bovine Anatomy Axons Nerve Regeneration Cell biology medicine.anatomical_structure Neurology chemistry Optic nerve biology.protein sense organs Axotomy Orbit (anatomy) |
| Zdroj: | Experimental Neurology. 107:214-221 |
| ISSN: | 0014-4886 |
| DOI: | 10.1016/0014-4886(90)90138-i |
| Popis: | One optic nerve of an adult mouse was crushed in the orbit. After 8 days, both retinas were explanted onto laminin-coated coverslips. Within 24 h, neurites grew out onto this substrate from explants with prior crush and by 48 h two-thirds of explants had neurites. On polylysine or plain glass, outgrowth was generally not seen until 48 h and this was significantly reduced compared to laminin. Explants without optic nerve crush did not extend neurites until about 5 days later. We suggest that axotomy induces a time-dependent regenerative response in adult mammalian retinal ganglion cells which includes the expression of laminin responsiveness. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|