Apple-Derived Pectin Modulates Gut Microbiota, Improves Gut Barrier Function, and Attenuates Metabolic Endotoxemia in Rats with Diet-Induced Obesity
| Autor: | Shuai Chen, Xinying Wang, Jingcheng Bi, Feng Tian, Chao Wu, Qiucheng Lei, Tingting Jiang, Xuejin Gao, Hong Yu Wang, Bingxian Xie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine obesity Time Factors Gut flora Weight Gain Rats Sprague-Dawley Intestinal Mucosa Receptor apple-derived pectin gut microbiota gut barrier function metabolic endotoxemia Nutrition and Dietetics biology Intestines Cholesterol Malus Pectins Tumor necrosis factor alpha Inflammation Mediators medicine.symptom lcsh:Nutrition. Foods and food supply medicine.medical_specialty Firmicutes Hypercholesterolemia lcsh:TX341-641 Inflammation Diet High-Fat Permeability Article Tight Junctions 03 medical and health sciences Internal medicine medicine Animals Plants Medicinal Bacteria biology.organism_classification medicine.disease Obesity Endotoxemia Gastrointestinal Microbiome Disease Models Animal 030104 developmental biology Endocrinology Fruit Immunology Anti-Obesity Agents Weight gain Diet-induced obese Biomarkers Phytotherapy Food Science |
| Zdroj: | Nutrients; Volume 8; Issue 3; Pages: 126 Nutrients, Vol 8, Iss 3, p 126 (2016) Nutrients |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu8030126 |
| Popis: | This study was aimed at determining potential effects of apple-derived pectin on weight gain, gut microbiota, gut barrier and metabolic endotoxemia in rat models of diet-induced obesity. The rats received a standard diet (control; Chow group; n = 8) or a high-fat diet (HFD; n = 32) for eight weeks to induce obesity. The top 50th percentile of weight-gainers were selected as diet induced obese rats. Thereafter, the Chow group continued on chow, and the diet induced obese rats were randomly divided into two groups and received HFD (HF group; n = 8) or pectin-supplemented HFD (HF-P group; n = 8) for six weeks. Compared to the HF group, the HF-P group showed attenuated weight gain (207.38 ± 7.96 g vs. 283.63 ± 10.17 g, p < 0.01) and serum total cholesterol level (1.46 ± 0.13 mmol/L vs. 2.06 ± 0.26 mmol/L, p < 0.01). Compared to the Chow group, the HF group showed a decrease in Bacteroidetes phylum and an increase in Firmicutes phylum, as well as subordinate categories (p < 0.01). These changes were restored to the normal levels in the HF-P group. Furthermore, compared to the HF group, the HF-P group displayed improved intestinal alkaline phosphatase (0.57 ± 0.20 vs. 0.30 ± 0.19, p < 0.05) and claudin 1 (0.76 ± 0.14 vs. 0.55 ± 0.18, p < 0.05) expression, and decreased Toll-like receptor 4 expression in ileal tissue (0.76 ± 0.58 vs. 2.04 ± 0.89, p < 0.01). The HF-P group also showed decreased inflammation (TNFα: 316.13 ± 7.62 EU/mL vs. 355.59 ± 8.10 EU/mL, p < 0.01; IL-6: 51.78 ± 2.35 EU/mL vs. 58.98 ± 2.59 EU/mL, p < 0.01) and metabolic endotoxemia (2.83 ± 0.42 EU/mL vs. 0.68 ± 0.14 EU/mL, p < 0.01). These results suggest that apple-derived pectin could modulate gut microbiota, attenuate metabolic endotoxemia and inflammation, and consequently suppress weight gain and fat accumulation in diet induced obese rats. |
| Databáze: | OpenAIRE |
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