Anti-proliferation and radiosensitization effects of chitooligosaccharides on human lung cancer line HepG2
| Autor: | Yan-Fen Xing, Fu-Shi Han, Bo-Han Cui, Xiao-Fang You, Xi-Wen Sun |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Medicine(all)
medicine.diagnostic_test Radiotherapy business.industry Cell Apoptosis General Medicine Cell cycle Molecular biology Chitooligosaccharides Flow cytometry medicine.anatomical_structure Cell culture medicine Viability assay Lung cancer Clonogenic assay business Cell survival curve Radiosensitization |
| Zdroj: | Asian Pacific Journal of Tropical Medicine. 8(9):757-761 |
| ISSN: | 1995-7645 |
| DOI: | 10.1016/j.apjtm.2015.07.025 |
| Popis: | ObjectiveTo observe the anti-proliferation and radiosensitization effect of chitooligosaccharides (COS) on human lung cancer cell line HepG2.MethodsCCK-8 assay was employed to obtain the inhibition ratio of COS on HepG2 cells at 24 h after treatment. The clonogenic assay was used to analyze the cell viability of RAY group and RAY + COS group with X-ray of 0, 1, 2, 4, 6 and 8 Gy, and the cell survival curve was used to analyze the sensitization ratio of COS. Flow cytometry was employed to detect cell cycle and apoptosis rate in control group, RAY group and RAY + COS group after 24 h treatment.ResultsCOS inhibited the proliferation of HepG2 cells, and the inhibition rate positively correlated with the concentration of COS. The cell viability decreased with increasing exposure dose in RAY group and RAY + COS group. The cell viabilities of RAY + COS group were lower than those of RAY group at the dose of 4, 6 and 8 Gy (P |
| Databáze: | OpenAIRE |
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